Bjelfman C, Hedborg F, Johansson I, Nordenskjöld M, Påhlman S
Department of Pathology, University of Uppsala, Sweden.
Cancer Res. 1990 Nov 1;50(21):6908-14.
The expression of the protooncogene c-src has been studied in specimens of childhood tumors with special reference to neuroblastoma and other tumors of neuronal origin. For comparison c-src gene expression was studied in seven neuroblastoma and neuroepithelioma cell lines. The structurally distinct neuronal product of the gene, pp60c-srN, expressed during normal development in neuroblasts and neurons, was identified by immunoblotting technique together with the fibroblast form, pp60c-src. While pp60c-src was found in most tumors studied, the neuronal form was restricted to neuroblastomas (23 of 27) and retinoblastomas (3 of 3) and could not be detected in the other childhood tumors. A dominance of the neuronal form, pp60c-srcN, was exclusively found in the infant cases of neuroblastoma (9 of 12), estimated to have good prognosis. These results indicate that pp60c-srcN might be a diagnostic marker in primitive childhood tumors. When expressed in higher amounts than pp60c-src, pp60c-srcN may be a positive prognostic marker in neuroblastoma, especially useful in the evaluation of infants. In addition, lack of pp60c-srcN seems to be incompatible with low stage neuroblastoma.
已对儿童肿瘤标本中的原癌基因c-src表达进行了研究，特别关注神经母细胞瘤和其他神经源性肿瘤。作为比较，对7种神经母细胞瘤和神经上皮瘤细胞系中的c-src基因表达进行了研究。通过免疫印迹技术，连同成纤维细胞形式的pp60c-src一起，鉴定出该基因在神经母细胞和神经元正常发育过程中表达的结构不同的神经元产物pp60c-srN。虽然在大多数研究的肿瘤中发现了pp60c-src，但神经元形式仅限于神经母细胞瘤（27例中的23例）和视网膜母细胞瘤（3例中的3例），在其他儿童肿瘤中未检测到。仅在估计预后良好的婴儿神经母细胞瘤病例（12例中的9例）中发现神经元形式的pp60c-srcN占主导地位。这些结果表明，pp60c-srcN可能是儿童原发性肿瘤的诊断标志物。当pp60c-srcN的表达量高于pp60c-src时，它可能是神经母细胞瘤的阳性预后标志物，对婴儿评估特别有用。此外，缺乏pp60c-srcN似乎与低分期神经母细胞瘤不相容。
