Wilsher S, Kölling M, Allen W R
University of Cambridge, Department of Veterinary Medicine Equine Fertility Unit, Mertoun Paddocks, Newmarket, Suffolk, UK.
Equine Vet J. 2006 Sep;38(5):428-32. doi: 10.2746/042516406778400547.
A level of synchrony between embryo and uterine environment is essential for the establishment of pregnancy when performing embryo transfer. The ability to extend the acceptable degree of asynchrony would allow more efficient use of recipient mares.
To establish if administration of the prostaglandin synthetase inhibitor, meclofenamic acid, to asynchronous recipient mares could widen the acceptable window of asynchrony for embryo transfer.
The prostaglandin synthetase inhibitory action of meclofenamic acid may act to suppress luteolysis and thereby allow for a greater degree of asynchrony between donor and recipient mares.
A total of 72 Grade 1 horse embryos were transferred nonsurgically into the uteri of recipient mares that had ovulated 2 (n = 20), 3 (n = 20), 4 (n = 16) or 5 (n = 16) days before the donor. Half of the mares in each group were treated orally with 1 g meclofenamic acid, beginning on Day 9 after ovulation and continuing for 7 days after embryo transfer.
Comparison of recipient:donor asynchrony between treated and untreated mares was: +2 days, 9/10 pregnancies vs. 8/10 (P= 1.00); by +3 days, 8/10 vs. 2/10 (P= 0.025); by +4 days, 5/8 vs. 1/8 (P = 0.121); and by +5 days 3/8 vs. 0/8 (P = 0.20). In 10/11 meclofenamic acid-treated and 23/25 untreated recipient mares that failed to become pregnant, luteolysis occurred at the normal time (14-19 days) after ovulation.
Treatment with meclofenamic acid supported the establishment of pregnancy in recipient mares that ovulated before the donors. However, meclofenamic acid appeared to provide this support in a manner other than by suppression of luteolysis.
Pregnancy rates can be increased in recipient mares that ovulate 3 days before the donor by administration of meclofenamic acid.
在进行胚胎移植时，胚胎与子宫环境之间的同步水平对于妊娠的建立至关重要。扩大可接受的不同步程度的能力将使受体母马得到更有效的利用。
确定对不同步的受体母马施用前列腺素合成酶抑制剂甲氯芬那酸是否能扩大胚胎移植可接受的不同步窗口期。
甲氯芬那酸的前列腺素合成酶抑制作用可能会抑制黄体溶解，从而使供体和受体母马之间存在更大程度的不同步。
总共72枚1级马胚胎通过非手术方式移植到排卵时间比供体母马早2天（n = 20）、3天（n = 20）、4天（n = 16）或5天（n = 16）的受体母马子宫内。每组中一半的母马在排卵后第9天开始口服1克甲氯芬那酸，并在胚胎移植后持续7天。
经治疗和未经治疗的母马中受体与供体不同步情况的比较为：相差2天时，妊娠率为9/10 对比 8/10（P = 1.00）；相差3天时，8/10 对比 2/10（P = 0.025）；相差4天时，5/8 对比 1/8（P = 0.121）；相差5天时，3/8 对比 0/8（P = 0.20）。在10/11经甲氯芬那酸治疗且未怀孕的受体母马和23/25未经治疗的受体母马中，黄体溶解在排卵后正常时间（14 - 19天）发生。
甲氯芬那酸治疗有助于排卵时间早于供体的受体母马妊娠的建立。然而，甲氯芬那酸似乎并非通过抑制黄体溶解的方式提供这种支持。
通过施用甲氯芬那酸，排卵时间比供体早3天的受体母马的妊娠率可以提高。
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