Camillo F, Cela M, Vannozzi I, Romagnoli S, Aria G
Istituto di Patologia Speciale e Clinica Chirurgica Veterinaria, Università di Pisa, Italy.
Equine Vet J Suppl. 1997 Dec(25):77-9. doi: 10.1111/j.2042-3306.1997.tb05106.x.
Fourteen normal, cyclic mares, treated to synchronise oestrus and ovulation and inseminated artificially with fresh semen, were assigned to a donor or a recipient group after ovulation, with the aim of obtaining a degree of synchrony of > or =2 days. Ten embryos, collected on Day 6 or 7 after ovulation (Day 0), were transferred nonsurgically to inseminated recipient mares (IRM) that had ovulated up to 5 days after the respective donors, or to pregnant recipient mares (PRM) that had ovulated 2-7 days before the donors. Embryonic size and development, as determined by ultrasound examination, were used to distinguish embryos derived from the recipient (recipient embryo = RE) or from the donor (transferred embryo = TE) mare. In cases of twin pregnancy, the RE was manually squeezed on Days 14-16. Abortion was induced in all mares on Day 30. Three of 6 TE developed in IRM. Two of 6 IRM developed a twin pregnancy (RE+TE), while 4 of 6 IRM developed a singleton pregnancy (1 TE and 3 RE). None of 4 TE developed in Day 9-14 PRM and one of these PRM lost her own embryo following ET. The experiment demonstrated that a mare can carry her own embryo and a transferred embryo simultaneously. However, the status of pregnancy does not improve conception rate in recipient mares that ovulate prior to the donor.
十四匹正常的、处于发情周期的母马，经过处理使其发情和排卵同步，并用新鲜精液进行人工授精，在排卵后被分为供体组或受体组，目的是获得大于或等于2天的同步程度。在排卵后第6天或第7天（第0天）收集到的10个胚胎，通过非手术方式移植到在各自供体排卵后最多5天排卵的授精受体母马（IRM），或移植到在供体排卵前2至7天排卵的怀孕受体母马（PRM）。通过超声检查确定的胚胎大小和发育情况，被用于区分源自受体（受体胚胎=RE）或供体（移植胚胎=TE）母马的胚胎。在怀有双胞胎的情况下，在第14至16天手动挤压RE。在第30天对所有母马进行引产。6个TE中有3个在IRM中发育。6个IRM中有2个怀有双胞胎（RE+TE），而6个IRM中有4个怀有单胎（1个TE和3个RE）。4个TE在第9至14天的PRM中均未发育，其中1个PRM在胚胎移植后失去了自己的胚胎。该实验表明，母马可以同时怀有自己的胚胎和移植的胚胎。然而，怀孕状态并未提高在供体之前排卵的受体母马的受孕率。
