Slack P M, Dayan A D, Slavin G, Tyrrell D A
Br J Exp Pathol. 1975 Aug;56(4):377-87.
Cell strains were established in culture from fragments of the brain from 2 cases each of Jakob-Creutzfeldt (JC) disease and subacute sclerosing panencephalitis (SSPE). After about 12 weeks strains from the former spontaneously formed persistent heaped up nodules of cells which appeared to produce reticulin-like fibrils as well as confluent sheets of rounded and spindle, fibroblast-like cells. Similar sheets of cells were obtained from the cases of SSPE but the only nodules formed were smaller and ephemeral. Attempts to detect virus in all 4 strains were made by inoculation of supernatant fluids into cultures of other laboratory cells, haemadsorption, co-cultivation, electron microscopy and immunofluorescence, and testing for interferon production. No evidence was found by any of these methods of the persistent presence of virus in the strains. Immunofluorescence revealed a probable anti-glial cell IgM autoantibody in one case of JC disease. Morphologically some cells resembled astrocytes and others fibroblasts. Those from JC disease contained more vacuoles and redundant membranes than did those from the cases of SSPE, features that are particularly striking in brain cells in human and animal cases of the spongiform encephalopathies.
从2例亚急性硬化性全脑炎（SSPE）和2例雅各布-克雅氏病（JC）患者的脑碎片中培养建立了细胞株。大约12周后，来自前者的细胞株自发形成了持续堆积的细胞结节，这些结节似乎产生了网状纤维样原纤维以及由圆形和梭形的成纤维细胞样细胞组成的融合片层。从SSPE患者的病例中也获得了类似的细胞片层，但形成的唯一结节较小且短暂。通过将上清液接种到其他实验室细胞培养物中、血细胞吸附、共培养、电子显微镜检查和免疫荧光检查以及检测干扰素产生，对所有4种细胞株进行了病毒检测。通过这些方法均未发现病毒在细胞株中持续存在的证据。免疫荧光检查在1例JC病病例中发现了一种可能的抗神经胶质细胞IgM自身抗体。形态学上，一些细胞类似于星形胶质细胞，另一些类似于成纤维细胞。与SSPE病例相比，JC病患者的细胞含有更多的空泡和多余的膜，这些特征在人类和动物海绵状脑病的脑细胞中尤为显著。
