通过逆转录聚合酶链反应（RT-PCR）检测球虫感染对SPF鸡淋巴组织中疫苗和vvIBDV的影响。
Impact of coccidial infection on vaccine- and vvIBDV in lymphoid tissues of SPF chickens as detected by RT-PCR.
作者信息
Kabell Susanne, Handberg Kurt J, Bisgaard Magne
机构信息
Danish Institute for Food and Veterinary Research, Hangøvej 2, DK-8200 Aarhus N, Denmark.
出版信息
Acta Vet Scand. 2006 Sep 4;48(1):17. doi: 10.1186/1751-0147-48-17.
BACKGROUND
This study aimed at investigating a potential effect caused by coccidia on the immune response to vaccine- and very virulent infectious bursal disease virus (vvIBDV) in SPF chickens.
METHODS
Two groups of three weeks old SPF chickens were vaccinated prior to inoculation with coccidia and challenge with virulent IBDV, all within a period of eight days. Two control groups were similarly treated, except that challenge with field virus was omitted in one group while inoculation with coccidia was omitted in the other group. Clinical signs, lesions in the intestines caused by coccidia, lesions in the bursa of Fabricius caused by IBDV, IBDV-antibody titres, and virus detection by reverse transcription polymerase chain reaction (RT-PCR) were compared among the groups. Lymphoid tissues and swab samples were analysed by general RT-PCR, and positive results were identified by strain specific duplex (DPX) RT-PCR.
RESULTS
In the triple-infected groups, vaccine strain IBDV was detected in spleen and thymus tissues, and no field virus was detected in bursa samples, contrary to the double-infected groups.
CONCLUSION
The results suggest an enhancing effect on the immune response caused by subclinical coccidiosis and vvIBDV acting in concert.
背景
本研究旨在调查球虫对SPF鸡针对疫苗和超强毒传染性法氏囊病病毒（vvIBDV）免疫反应的潜在影响。
方法
两组三周龄的SPF鸡在接种球虫和强毒IBDV之前进行疫苗接种，所有操作均在八天内完成。两个对照组接受类似处理，只是一组省略了田间病毒攻毒，另一组省略了球虫接种。比较各实验组的临床症状、球虫引起的肠道病变、IBDV引起的法氏囊病变、IBDV抗体效价以及通过逆转录聚合酶链反应（RT-PCR）进行的病毒检测。通过常规RT-PCR分析淋巴组织和拭子样本，并通过菌株特异性双重（DPX）RT-PCR鉴定阳性结果。
结果
与双重感染组相反，在三重感染组中，在脾脏和胸腺组织中检测到疫苗株IBDV，在法氏囊样本中未检测到田间病毒。
结论
结果表明亚临床球虫病和vvIBDV协同作用对免疫反应有增强作用。
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