Alzhanova Dina, Hruby Dennis E
Oregon State University, Department of Microbiology, 220 Nash Hall, Corvallis, OR 97331-3804, USA.
J Virol. 2006 Dec;80(23):11520-7. doi: 10.1128/JVI.00287-06. Epub 2006 Sep 20.
Poxviruses are the only DNA viruses known to replicate and assemble in the cytoplasm of infected cells. Poxvirus morphogenesis is a complicated process in which four distinct infectious forms of the virus are produced: intracellular mature virus, intracellular enveloped virus, cell-associated enveloped virus, and extracellular enveloped virus. The source of primary membrane wrapping the intracellular mature virus, the first infectious form, is still unknown. Although the membrane was suggested to originate from the endoplasmic reticulum-Golgi intermediate compartment, none of the marker proteins from this or any other cell compartments has been found in the intracellular mature virus. Thus, it was hypothesized that the membrane is either extensively modified by the virus or synthesized de novo. In the work described here, we demonstrate that a host cell protein residing in the trans-Golgi network membrane, golgin-97, is transported to the sites of virus replication and assembly and becomes incorporated into the virions during poxvirus infection. Inside the virion, golgin-97 is associated with the insoluble core protein fraction. Being able to adopt a long rod-like structure, the protein apparently extends through the virion envelope and protrudes from its surface. Here we discuss the potential role and functions of golgin-97 in poxvirus replication and propose two working models.
痘病毒是已知唯一能在受感染细胞的细胞质中进行复制和组装的DNA病毒。痘病毒形态发生是一个复杂的过程，在此过程中会产生四种不同的病毒感染形式：细胞内成熟病毒、细胞内包膜病毒、细胞相关包膜病毒和细胞外包膜病毒。包裹第一种感染形式即细胞内成熟病毒的原始膜的来源仍然未知。尽管有人认为该膜起源于内质网-高尔基体中间区室，但在细胞内成熟病毒中未发现来自该区室或任何其他细胞区室的标记蛋白。因此，有人推测该膜要么被病毒广泛修饰，要么是重新合成的。在本文所述的研究中，我们证明了一种存在于反式高尔基体网络膜中的宿主细胞蛋白——高尔基体蛋白97，在痘病毒感染期间被转运到病毒复制和组装位点，并被整合到病毒粒子中。在病毒粒子内部，高尔基体蛋白97与不溶性核心蛋白部分相关联。该蛋白能够形成长杆状结构，显然穿过病毒粒子包膜并从其表面突出。在此我们讨论了高尔基体蛋白97在痘病毒复制中的潜在作用和功能，并提出了两个工作模型。
