癌症研究中的系统生物学：从基因组学到细胞组学

Stilwell Jackie L, Guan Yinghui, Neve Richard M, Gray Joe W

Lawrence Berkeley National Laboratory, Berkeley, CA, USA.

Methods Mol Biol. 2007;356:353-65. doi: 10.1385/1-59745-217-3:353.

Cancers result from large-scale deregulation of genes that lead to cancer pathophysiologies such as increase proliferation, decreased apoptosis, increased motility, increased angiogenesis, and others. Genes that influence proliferation and apoptosis are particularly attractive as therapeutic targets. To identify genes that influence these phenotypes, we have developed simple and rapid methods to measure apoptosis and cell proliferation using high content screening with YO-PRO-1 and anti-BrdU staining of BrdU pulsed cells, respectively.

癌症是由基因的大规模失调引起的，这些失调会导致癌症病理生理过程，如增殖增加、细胞凋亡减少、迁移能力增强、血管生成增加等。影响增殖和细胞凋亡的基因作为治疗靶点特别具有吸引力。为了鉴定影响这些表型的基因，我们分别开发了简单快速的方法，通过使用YO-PRO-1进行高内涵筛选以及对BrdU脉冲处理的细胞进行抗BrdU染色来测量细胞凋亡和细胞增殖。