Khan K M, Hatfield J S, Drescher D G
Department of Otolaryngology, Wayne State University School of Medicine, Detroit, Michigan 48201.
J Histochem Cytochem. 1990 Nov;38(11):1615-23. doi: 10.1177/38.11.1698852.
The surface of most cells is covered by glycoconjugates. The composition and thickness of the surface coat varies among different cell types. The purpose of the present study was to demonstrate the presence of and to characterize the cell coat surrounding the cells in the saccular macula of the rainbow trout. Tissues were fixed in Karnovsky's fixative containing either ruthenium red (0.5, 1, or 2%) or tannic acid (1, 2, or 4%). The apical surface of the sensory and supporting cells reacted with both agents. Varying the concentration of the compounds within a certain range did not significantly affect the degree of tissue staining. Whereas ruthenium red staining was distributed evenly along the luminal surface of the epithelium and along the length of the stereocilia, tannic acid formed electron-dense clumps on the luminal surface of sensory and non-sensory cells and in the basal region of the macular epithelium. The stereocilia of the sensory cells also exhibited tannic acid-positive, electrondense precipitate, particularly near the distal ends of these processes, while uniform staining of the plasma membrane was seen along their lengths. The results of this study suggest that the trout saccular macula is provided with extracellular microenvironments which may be necessary for functional integrity.
大多数细胞的表面都覆盖着糖缀合物。表面被膜的组成和厚度在不同细胞类型之间有所不同。本研究的目的是证明虹鳟鱼球囊斑细胞周围细胞被膜的存在并对其进行表征。组织用含有钌红（0.5%、1%或2%）或单宁酸（1%、2%或4%）的卡诺夫斯基固定液固定。感觉细胞和支持细胞的顶端表面与这两种试剂都发生反应。在一定范围内改变化合物的浓度对组织染色程度没有显著影响。钌红染色沿上皮细胞腔面和静纤毛长度均匀分布，而单宁酸在感觉和非感觉细胞的腔面以及黄斑上皮的基部区域形成电子致密团块。感觉细胞的静纤毛也呈现单宁酸阳性电子致密沉淀物，特别是在这些突起的远端附近，而沿其长度可见质膜均匀染色。本研究结果表明，虹鳟鱼球囊斑具有细胞外微环境，这可能对功能完整性是必要的。
