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钌红染色显示的内耳感觉细胞和支持细胞的细胞被膜。

The cell coat of inner ear sensory and supporting cells as demonstrated by ruthenium red.

作者信息

Slepecky N, Chamberlain S C

出版信息

Hear Res. 1985 Mar;17(3):281-8. doi: 10.1016/0378-5955(85)90072-3.

DOI:10.1016/0378-5955(85)90072-3
PMID:2410399
Abstract

The ultrastructure of the cell coat of sensory and supporting cells of chinchilla and lizard inner ears was studied using ruthenium red. On the apical surface of both cell types, in both animals, the glycoproteins in the cell coat stain positively with this cationic dye. The apical surface of the sensory hair cells displays no regional variations in cell coat thickness. The uniform staining along the length of the stereocilia is not influenced by the normal presence or absence of a tectorial membrane. Although no micro-domains in the glycoproteins that stain positively with ruthenium red were observed that might correlate with the ultrastructural localization of sites of initiation of the transduction event, the cell coat material on the apical cell surface might play an important role in sequestering ions (particularly calcium) which are required for the transduction process.

摘要

利用钌红对毛丝鼠和蜥蜴内耳感觉细胞与支持细胞的细胞衣超微结构进行了研究。在这两种动物的这两种细胞类型的顶端表面,细胞衣中的糖蛋白用这种阳离子染料染色呈阳性。感觉毛细胞的顶端表面在细胞衣厚度上没有区域差异。沿静纤毛长度的均匀染色不受盖膜正常存在与否的影响。虽然未观察到用钌红染色呈阳性的糖蛋白中的微结构域可能与转导事件起始位点的超微结构定位相关,但顶端细胞表面的细胞衣物质可能在隔离转导过程所需的离子(特别是钙)方面发挥重要作用。

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