针对人促甲状腺激素不同表位的单克隆抗体的制备与特性鉴定

Production and characterisation of monoclonal antibodies directed against different epitopes of human thyroid stimulating hormone.

作者信息

Braun J M, Charreire J

机构信息

INSERM U. 283, Hôpital Cochin, Paris, France.

出版信息

J Immunol Methods. 1990 Sep 14;132(2):197-203. doi: 10.1016/0022-1759(90)90030-y.

DOI:10.1016/0022-1759(90)90030-y

PMID:1698874

Abstract

We have produced monoclonal antibodies (mAbs) to human thyroid stimulating hormone (hTSH) and selected five that specifically recognize hTSH and do not cross-react with the other human glycoprotein hormones such as luteinizing hormone (LH), chorionic gonadotropin (CG), follicle stimulating hormone (FSH). All of the antibodies were of the IgG1 subclass with affinities ranging from 5.3 X 10(8) to 1.9 X 10(10) mol-1.l; they could be assigned to two subgroups on the basis of their epitope specificity.

摘要

我们制备了针对人促甲状腺激素（hTSH）的单克隆抗体（mAb），并筛选出5种能特异性识别hTSH且不与其他人类糖蛋白激素（如促黄体生成素（LH）、绒毛膜促性腺激素（CG）、促卵泡激素（FSH））发生交叉反应的抗体。所有抗体均为IgG1亚类，亲和力范围为5.3×10⁸至1.9×10¹⁰mol⁻¹·L；根据其表位特异性可将它们分为两个亚组。