Soos M, Siddle K
J Immunol Methods. 1982;51(1):57-68. doi: 10.1016/0022-1759(82)90382-9.
Monoclonal antibodies directed against human thyroid stimulating hormone (TSH) were obtained from hybrid myelomas, following fusion of mouse NSI myeloma cells with mouse spleen cells. Ten different antibodies were obtained from 4 separate fusions. Eight antibodies were of the IgG1 subclass. Affinities of antibodies for TSH were in the range 2 x 10(8)-5 x 10(10) M-1. Five of the antibodies were specific for TSH and did not react with LH, FSH or hCG. The remaining antibodies reacted with all these hormones and were assumed to recognise their common (alpha) subunit. The 5 specific antibodies fell into 3 subgroups recognising distinct antigenic determinants, whereas the 5 non-specific antibodies recognised a single determinant or closely related set of sites. It is concluded that these antibodies should be valuable reagents for use in sensitive and specific two-site immunoradiometric assays.
通过将小鼠NSI骨髓瘤细胞与小鼠脾细胞融合，从杂交骨髓瘤中获得了针对人促甲状腺激素（TSH）的单克隆抗体。从4次单独融合中获得了10种不同的抗体。8种抗体属于IgG1亚类。抗体对TSH的亲和力在2×10⁸ - 5×10¹⁰ M⁻¹范围内。其中5种抗体对TSH具有特异性，不与LH、FSH或hCG发生反应。其余抗体与所有这些激素发生反应，被认为识别它们的共同（α）亚基。5种特异性抗体分为3个亚组，识别不同的抗原决定簇，而5种非特异性抗体识别单个决定簇或一组密切相关的位点。得出的结论是，这些抗体应是用于灵敏和特异的双位点免疫放射分析的有价值试剂。
