Jaegle M, Devic M, Longstaff M, Baulcombe D
Department of Molecular Genetics, Institute of Plant Science Research, Trumpington, Cambridge, U.K.
J Gen Virol. 1990 Sep;71 ( Pt 9):1905-12. doi: 10.1099/0022-1317-71-9-1905.
Plants infected with cucumber mosaic virus (CMV) (KIN strain) produce a mild mosaic disease on tobacco whereas infections of CMV with satellite RNA (strain Y) cause a severe yellow mosaic. Analysis of recombinant and mutant forms of satellite RNA identified a site (nucleotides 185/186) in the Y satellite RNA that affects the ability to induce the yellow mosaic in combination with CMV but not with tomato aspermy virus. The location of this site with respect to other mutations in the satellite RNA indicated that polypeptides, which may be encoded by the satellite RNA, have no role in induction of yellow mosaic symptoms. The symptom induction is therefore an effect of the satellite RNA on the host plant with the intervention of the helper virus. In the course of the mutation analysis of satellite RNA we detected several secondary mutations which arose in planta. Two of these were deletions of more than 80 nucleotides. Other forms of mutant satellite RNA were non-functional even though the modifications involved nucleotides completely within the large secondary deletions. These data imply complex intramolecular interactions in the satellite RNA.
感染黄瓜花叶病毒（CMV）（KIN株系）的植物在烟草上产生轻度花叶病，而感染带有卫星RNA（Y株系）的CMV会导致严重的黄色花叶病。对卫星RNA的重组和突变形式进行分析，在Y卫星RNA中确定了一个位点（核苷酸185/186），该位点影响与CMV共同诱导黄色花叶病的能力，但与番茄不孕病毒共同作用时则不影响。该位点相对于卫星RNA中其他突变的位置表明，卫星RNA可能编码的多肽在诱导黄色花叶病症状方面没有作用。因此，症状诱导是卫星RNA在辅助病毒的干预下对宿主植物产生的一种影响。在卫星RNA的突变分析过程中，我们检测到了一些在植物体内出现的二级突变。其中两个是超过80个核苷酸的缺失。其他形式的突变卫星RNA没有功能，尽管这些修饰涉及的核苷酸完全在大的二级缺失范围内。这些数据表明卫星RNA中存在复杂的分子内相互作用。
