一种用于检测人心脏肌钙蛋白T的表面等离子体共振免疫传感器，该传感器利用抗生物素蛋白与生物素在羧甲基葡聚糖修饰的金芯片上的特异性结合。
An SPR immunosensor for human cardiac troponin T using specific binding avidin to biotin at carboxymethyldextran-modified gold chip.
作者信息
Dutra Rosa Fireman, Kubota Lauro Tatsuo
机构信息
Departamento de Patologia, Instituto de Ciências Biológicas, Universidade de Pernambuco, Recife, Brasil, Rua Arnóbio Marques, 310, Santo Amaro, 50100-130. Recife, PE , Brazil.
出版信息
Clin Chim Acta. 2007 Feb;376(1-2):114-20. doi: 10.1016/j.cca.2006.07.029. Epub 2006 Aug 5.
BACKGROUND
The cardiac troponin T (TnT) is a cardiospecific, highly sensitive marker for myocardial damage and is immediately released to bloodstream during the acute myocardial infarction (AMI). Surface plasmon resonance (SPR) immunosensor was developed for the quick detection of human cTnT in real time.
METHODS
An SPR sensor streptavidin terminated self-assembled monolayer, which was used to binding biotinylated anti troponin T monoclonal antibodies, was developed.
RESULTS
The cTnT was determined from successive injections with a linear range from 0.03 up to 6.5 ng/ml. The detection limit was 0.01 ng/ml corresponding to a resonant angle change of 1.28 millidegrees. The system presented a good repeatability with 3.4% of variation between run after regeneration of the coated surface with a solution of 1% SDS. The effects of blocking of non-specific adsorption using different solutions were studied. It was possible to measure the cTnT without dilution of the human serum with good specificity and reproducibility.
CONCLUSION
This sensor is practical and offer quick response in interval of 800 s.
背景
心肌肌钙蛋白T（TnT）是心肌损伤的心脏特异性、高敏标志物，在急性心肌梗死（AMI）期间会立即释放到血液中。表面等离子体共振（SPR）免疫传感器被开发用于实时快速检测人cTnT。
方法
开发了一种SPR传感器，其链霉亲和素终止的自组装单分子层用于结合生物素化的抗肌钙蛋白T单克隆抗体。
结果
通过连续注射测定cTnT，线性范围为0.03至6.5 ng/ml。检测限为0.01 ng/ml，对应于1.28毫度的共振角变化。用1% SDS溶液再生包被表面后，该系统具有良好的重复性，每次运行之间的变化为3.4%。研究了使用不同溶液封闭非特异性吸附的效果。无需稀释人血清即可测量cTnT，具有良好的特异性和重现性。
结论
该传感器实用，在800 s的间隔内提供快速响应。
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