Santana Gilvânia M, Silva Anne K S, Foguel Marcos V, Dutra Rosa F
Biomedical Engineering Laboratory, Federal University of Pernambuco, Av. Professor Moraes Rego, Recife, PE 50670-901 Brazil.
J Mater Sci. 2022;57(9):5586-5595. doi: 10.1007/s10853-022-06992-5. Epub 2022 Feb 26.
An ultrasensitive label-free electrochemical immunosensor was developed for hepatitis C antibodies (anti-HCV). Worldwide, it is estimated 71 million people have HCV infection in a chronic stage that may lead to cirrose and cancer. To achieve HCV elimination, health programs should include screening testing based on anti-HCV detection allowing the early-stage treatment. The immunosensor was based on a graphene oxide-polypyrrole (PPy-GO) film one-step electropolymerized on the electrode surface. Ultrasensitive anti-HCV detection was ensured by HCV antigen conjugated to biotin that was immobilized in a great amount on streptavidin-coated nanostructured surface. Analytical responses were obtained by anodic peaks from the square wave voltammetry in the presence of ferrocyanide/ferricyanide as a redox probe. This immunosensor exhibited a linear range from 2 to 14 ng mL of anti-HCV and a limit of detection in the clinical range (1.63 ng mL). Furthermore, the immunosensor presented an efficient performance for the determination of anti-HCV in spiked serum samples, becoming this developed nanosensor as potential tools for early HCV diagnosis and screening.
The online version contains supplementary material available at 10.1007/s10853-022-06992-5.
开发了一种用于丙型肝炎抗体（抗-HCV）的超灵敏无标记电化学免疫传感器。据估计，全球有7100万人处于慢性丙型肝炎病毒感染阶段，这可能会导致肝硬化和癌症。为了实现消除丙型肝炎病毒的目标，卫生项目应包括基于抗-HCV检测的筛查测试，以便早期治疗。该免疫传感器基于在电极表面一步电聚合的氧化石墨烯-聚吡咯（PPy-GO）薄膜。通过与生物素偶联的丙型肝炎病毒抗原确保了超灵敏的抗-HCV检测，该抗原大量固定在链霉亲和素包被的纳米结构表面。在存在亚铁氰化物/铁氰化物作为氧化还原探针的情况下，通过方波伏安法的阳极峰获得分析响应。该免疫传感器的抗-HCV线性范围为2至14 ng/mL，临床检测限为1.63 ng/mL。此外，该免疫传感器在加标血清样品中测定抗-HCV时表现出高效性能，使这种开发的纳米传感器成为早期丙型肝炎病毒诊断和筛查的潜在工具。
在线版本包含可在10.1007/s10853-022-06992-5获取的补充材料。
