Rogers Walter J, Meyer Craig H, Kramer Christopher M
Department of Radiology, University of Virginia Health System, Charlottesville, VA 22908, USA.
Nat Clin Pract Cardiovasc Med. 2006 Oct;3(10):554-62. doi: 10.1038/ncpcardio0659.
Animal studies have shown some success in the use of stem cells of diverse origins to treat heart failure and ventricular dysfunction secondary to ischemic injury. The clinical use of these cells is, therefore, promising. In order to develop effective cell therapies, the location, distribution and long-term viability of these cells must be evaluated in a noninvasive manner. MRI of cells labeled with magnetically visible contrast agents after either direct injection or local or intravenous infusion has the potential to fulfill this goal. In this Review, techniques for labeling and imaging a variety of cells will be discussed. Particular attention will be given to the advantages and limitations of various contrast agents and passive and facilitated cell-labeling methods, as well as to imaging techniques that produce negative and positive contrast, and the effect on image quantification of compartmentalization of contrast agents within the cell.
动物研究表明，使用多种来源的干细胞治疗缺血性损伤继发的心力衰竭和心室功能障碍已取得一定成效。因此，这些细胞在临床上的应用前景广阔。为了开发有效的细胞疗法，必须以非侵入性方式评估这些细胞的位置、分布和长期存活率。直接注射或局部或静脉输注后，用磁性可视造影剂标记细胞的MRI有潜力实现这一目标。在本综述中，将讨论标记和成像各种细胞的技术。将特别关注各种造影剂以及被动和促进性细胞标记方法的优缺点，以及产生阴性和阳性对比的成像技术，以及造影剂在细胞内的分隔对图像定量的影响。
