在硫酸鱼精蛋白存在的情况下，通过电穿孔用磁共振成像造影剂进行快速高效的细胞标记。

Rapid and efficient cell labeling with a MRI contrast agent by electroporation in the presence of protamine sulfate.

作者信息

Wang Lijun, Wang Zhigang, Frank Tim G, Brown Stuart I, Chudek Sandy A, Cuschieri Alfred

机构信息

Institute for Medical Science & Technology (IMSaT), School of Engineering, Physics & Mathematics, University of Dundee, Dundee, UK.

出版信息

Nanomedicine (Lond). 2009 Apr;4(3):305-15. doi: 10.2217/nnm.09.6.

DOI:10.2217/nnm.09.6

PMID:19331538

Abstract

AIMS

To investigate various protocols for magnetic labeling of human cancer cells with ferumoxides with a view to developing an effective and fast technique for potential clinical use in MRI.

MATERIALS & METHODS: Transfection methods utilizing poly-L-lysine and protamine sulfate (PS), electroporation, and combination of PS with electroporation were evaluated in this in vitro study.

RESULTS

Although transfection was more effective in terms of uptake rates (95-100%) and intracellular iron concentrations (4.01-7.34 pg/cell), all transfection agents required prolonged incubation. By contrast, electroporation yielded fast labeling but with a lower efficacy (68-75%, 1.63-2.59 pg/cell). The addition of PS to electroporation increased the labeling efficacy (80-91%, 2.84-4.16 pg/cell) and protected cell viability. This combined method also resulted in the best T(2)*-shortening effect in the in vitro cellular MRI.

CONCLUSION

The combined PS-electroporation method provides a fast and efficient protocol for ferumoxide-based cellular imaging and therapeutic procedure.

摘要

目的

研究使用超顺磁性氧化铁对人癌细胞进行磁性标记的各种方案，以期开发出一种有效且快速的技术，用于磁共振成像的潜在临床应用。

材料与方法

在这项体外研究中，评估了利用聚-L-赖氨酸和硫酸鱼精蛋白（PS）的转染方法、电穿孔法以及PS与电穿孔相结合的方法。

结果

尽管就摄取率（95 - 100%）和细胞内铁浓度（4.01 - 7.34 pg/细胞）而言，转染更有效，但所有转染试剂都需要长时间孵育。相比之下，电穿孔产生快速标记，但效率较低（68 - 75%，1.63 - 2.59 pg/细胞）。在电穿孔中添加PS提高了标记效率（80 - 91%，2.84 - 4.16 pg/细胞）并保护了细胞活力。这种联合方法在体外细胞磁共振成像中也产生了最佳的T(2)*缩短效应。