Yoon Ju-Won, Kim Haeng-Hoon, Ko Ho-Cheol, Hwang Hae-Sung, Hong Eun-Sun, Cho Eun-Gi, Engelmann Florent
National Institute of Agricultural Biotechnology, RDA, Suwon, Korea.
Cryo Letters. 2006 Jul-Aug;27(4):211-22.
In this paper, we studied the effect of subculture of mother-plants and of preculture of shoot tips of two potato varieties (Dejima, cultivated and STN13, wild) cryopreserved using the droplet-vitrification technique. The subculture conditions (light intensity, aeration and planting density) significantly affected survival of both non-cryopreserved and cryopreserved shoot-tips in both varieties. The subculture duration and the position of the shoot tips on the axis of the in vitro plantlets had a significant (P<0.0001) effect on survival of cryopreserved shoot tips. The optimal subculture duration was 7 and 5 weeks and the optimal size of shoot tips was 1.5-2.0 and 1.0-1.5 mm for var. Dejima and STN13, respectively. Survival of cryopreserved shoot tips was influenced by the sucrose concentration in the preculture medium and the preculture duration. The highest survival of cryopreserved shoot tips was observed after preculture with 0.3 M sucrose for 8 h followed by 0.7 M sucrose for 18 h. These results indicate that the parameters of the subculture of mother-plants and of preculture of shoot tips should be carefully optimized, especially in the case of wild species.
在本文中，我们研究了母株继代培养以及使用玻璃化法超低温保存的两个马铃薯品种（栽培品种德岛和野生品种STN13）茎尖预培养的效果。继代培养条件（光照强度、通气和种植密度）对两个品种未超低温保存和超低温保存的茎尖存活率均有显著影响。继代培养时间以及茎尖在离体苗轴上的位置对超低温保存茎尖的存活率有极显著影响（P<0.0001）。德岛品种和STN13品种继代培养的最佳时间分别为7周和5周，茎尖的最佳大小分别为1.5 - 2.0毫米和1.0 - 1.5毫米。超低温保存茎尖的存活率受预培养基中蔗糖浓度和预培养时间的影响。在0.3 M蔗糖预培养8小时，随后0.7 M蔗糖预培养18小时后，超低温保存茎尖的存活率最高。这些结果表明，母株继代培养和茎尖预培养的参数应仔细优化，尤其是对于野生种。
