Marco-Jimenez F, Lavara R, Vicente J S, Viudes-de-Castro M P
Animal Research and Technology Center. Valencian Institute of Agrarian Research. Segorbe 12400, Spain.
Cryo Letters. 2006 Jul-Aug;27(4):261-8.
This study researches the effects of supplementation with reduced glutathione (GSH, 0.5 mm) and oxidised glutathione (GSSG, 0.5 mM) freezing extenders on different semen parameters after equilibration with DMSO preservation solution (45 min at 5 degrees C) and post-thawing. The main findings that emerged from this study are that (i) addition of GSH and GSSG to the freezing media did not result in any improvement in functional sperm tests after equilibrium phase. (ii) No differences were observed after cryopreservation in functional sperm tests and embryo recovery rate. In conclusion, the addition of 0.5 mM GSH or GSSG appears not to play an important role in sperm antioxidant defence during cooling and freezing in rabbit spermatozoa.
本研究探讨了在二甲基亚砜保存溶液（5℃下45分钟）平衡及解冻后，添加还原型谷胱甘肽（GSH，0.5 mM）和氧化型谷胱甘肽（GSSG，0.5 mM）的冷冻稀释液对不同精液参数的影响。本研究的主要发现为：（i）在冷冻培养基中添加GSH和GSSG在平衡期后并未使功能性精子检测结果得到任何改善。（ii）冷冻保存后，功能性精子检测和胚胎回收率未观察到差异。总之，添加0.5 mM GSH或GSSG在兔精子冷却和冷冻过程中似乎对精子抗氧化防御不起重要作用。
