Gadea Joaquín, García-Vazquez Francisco, Matás Carmen, Gardón Juan C, Cánovas Sebastián, Gumbao David
Department of Physiology, Facultad de Veterinaria, Universidad de Murcia, 30100 Murcia, Spain.
J Androl. 2005 May-Jun;26(3):396-404. doi: 10.2164/jandrol.04155.
In this study, we evaluated the effects of glutathione (L-gamma-glutamyl-L-cysteinylglycine; GSH) supplementation of the freezing extender on semen parameters during the cooling (2 hours at 5 degrees C) and freezing phases of the cryopreservation process to compensate for the decrease in GSH content observed during sperm freezing. To fully address these questions, we incorporated a new set of functional sperm tests. These included tests of mitochondrial function, inducibility of the acrosome reaction, in vitro penetration (IVP) of oocytes, changes in sulfhydryl group content in membrane proteins, and capacitation status. The main findings emerging from this study were that the addition of GSH to the freezing media resulted in 1) an improvement in percent motility (%MOT) and motion parameters of thawed spermatozoa, as measured by both microscopic analysis and computer-assisted semen analysis (CASA); 2) a higher number of total viable spermatozoa; 3) a higher number of noncapacitated viable spermatozoa; and 4) a decrease in the number of spermatozoa with changes in the sulfhydryl groups in membrane proteins. This protective effect on sperm function was more pronounced with 1 mM of GSH than with 5 mM of GSH.
在本研究中，我们评估了在冷冻保存过程的冷却阶段（5℃下2小时）和冷冻阶段，向冷冻稀释液中添加谷胱甘肽（L-γ-谷氨酰-L-半胱氨酰甘氨酸；GSH）对精液参数的影响，以弥补精子冷冻过程中观察到的GSH含量下降。为了全面解决这些问题，我们纳入了一组新的功能性精子检测。这些检测包括线粒体功能检测、顶体反应诱导性检测、卵母细胞体外穿透（IVP）检测、膜蛋白中巯基含量变化检测以及获能状态检测。本研究得出的主要结果是，向冷冻培养基中添加GSH导致：1）通过显微镜分析和计算机辅助精液分析（CASA）测量，解冻精子的活力百分比（%MOT）和运动参数得到改善；2）总活精子数量增加；3）未获能活精子数量增加；4）膜蛋白中巯基发生变化的精子数量减少。1 mM GSH对精子功能的这种保护作用比5 mM GSH更明显。
