文献检索，用中文搜 PubMed

Suppr 超能文献

核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

J Physiol. 1967 Apr;189(3):461-73. doi: 10.1113/jphysiol.1967.sp008179.

Spectrophotometric measurements, using the rapid-mixing constantflow and stopped-flow techniques, have been made of the rate of egress of oxygen from human HbO(2) in solution and in erythrocytes.2. By 1:20 dilution in a medium containing a high concentration of carbon monoxide k(4), the rate constant of the reaction Hb(4)O(8) --> Hb(4)O(6)+O(2) has been determined. At pH 7.4 and 20 degrees C, k(4) in solution was 42.0 sec(-1) and within the intact cell 41.4 sec(-1). The respective activation energies were 19.3 and 18 kcal.3. The rate of dissociation of HbO(2) to Hb, after mixing an erythrocyte suspension with Na(2)S(2)O(4), was identical, over at least 50% of the reaction, with the change of HbO(2) to HbCO above.4. An analysis of the data indicates that the cell membrane has little or no resistance to the passage of oxygen and the internal contents are effectively mixed.

利用快速混合恒流和停流技术，通过分光光度法测量了溶液和红细胞中氧从人血红蛋白氧合（HbO₂）逸出的速率。
通过在含有高浓度一氧化碳k(4)的介质中按1:20稀释，测定了反应Hb₄O₈→Hb₄O₆ + O₂的速率常数。在pH 7.4和20℃时，溶液中的k(4)为42.0秒⁻¹，完整细胞内为41.4秒⁻¹。各自的活化能分别为19.3千卡和18千卡。
将红细胞悬液与Na₂S₂O₄混合后，HbO₂解离为Hb的速率，在至少50%的反应过程中，与上述HbO₂转变为HbCO的变化相同。
数据分析表明，细胞膜对氧的通透几乎没有阻力，且细胞内成分能有效混合。

J Physiol. 1967 Apr;189(3):461-73. doi: 10.1113/jphysiol.1967.sp008179.

Spectrophotometric measurements, using the rapid-mixing constantflow and stopped-flow techniques, have been made of the rate of egress of oxygen from human HbO(2) in solution and in erythrocytes.2. By 1:20 dilution in a medium containing a high concentration of carbon monoxide k(4), the rate constant of the reaction Hb(4)O(8) --> Hb(4)O(6)+O(2) has been determined. At pH 7.4 and 20 degrees C, k(4) in solution was 42.0 sec(-1) and within the intact cell 41.4 sec(-1). The respective activation energies were 19.3 and 18 kcal.3. The rate of dissociation of HbO(2) to Hb, after mixing an erythrocyte suspension with Na(2)S(2)O(4), was identical, over at least 50% of the reaction, with the change of HbO(2) to HbCO above.4. An analysis of the data indicates that the cell membrane has little or no resistance to the passage of oxygen and the internal contents are effectively mixed.

利用快速混合恒流和停流技术，通过分光光度法测量了溶液和红细胞中氧从人血红蛋白氧合（HbO₂）逸出的速率。
通过在含有高浓度一氧化碳k(4)的介质中按1:20稀释，测定了反应Hb₄O₈→Hb₄O₆ + O₂的速率常数。在pH 7.4和20℃时，溶液中的k(4)为42.0秒⁻¹，完整细胞内为41.4秒⁻¹。各自的活化能分别为19.3千卡和18千卡。
将红细胞悬液与Na₂S₂O₄混合后，HbO₂解离为Hb的速率，在至少50%的反应过程中，与上述HbO₂转变为HbCO的变化相同。
数据分析表明，细胞膜对氧的通透几乎没有阻力，且细胞内成分能有效混合。