Tsukise A, Yamada K
Department of Veterinary Anatomy, College of Agriculture and Veterinary Medicine, Nihon University, Kanagawa, Japan.
Acta Histochem. 1990;88(2):131-7. doi: 10.1016/S0065-1281(11)80125-X.
In the porcine prostate, glycoconjugates elaborated and released by the glandular cells have been studied by means of a series of histochemical methods of light microscopy. The employed methods included several routine procedures in combination with digestion by carbohydrate-degrading enzymes and peroxidase-labelled lectin diaminobenzidine procedures. In the glandular tissues, the particular glycoconjugates were shown to be secretory glycoproteins which contained vicinal diol, sulphate, and carboxyl groups and were provided with different saccharide residues such as alpha-D-mannose, alpha-D-glucose, lactose, N-acetyl-D-galactosamine, beta-D-galactose, alpha-L-fucose, N-acetyl-D-glucosamine, and N-acetyl-neuraminic acid. These glycoconjugates were, likewise, found to exhibit positive reaction for proteins. The present histochemical results have been discussed with special reference to the histophysiological functions performed by the prostate gland in the pig.
在猪前列腺中，已通过一系列光学显微镜组织化学方法对腺细胞分泌和释放的糖缀合物进行了研究。所采用的方法包括几种常规程序，结合碳水化合物降解酶消化和过氧化物酶标记凝集素二氨基联苯胺程序。在腺组织中，特定的糖缀合物显示为分泌性糖蛋白，其含有邻二醇、硫酸盐和羧基，并带有不同的糖残基，如α-D-甘露糖、α-D-葡萄糖、乳糖、N-乙酰-D-半乳糖胺、β-D-半乳糖、α-L-岩藻糖、N-乙酰-D-葡萄糖胺和N-乙酰神经氨酸。同样，这些糖缀合物也被发现对蛋白质呈阳性反应。本文结合猪前列腺的组织生理功能对当前的组织化学结果进行了讨论。
