Suprasert A, Fujioka T, Yamada K
Histochem J. 1986 Feb-Mar;18(2-3):115-21. doi: 10.1007/BF01675365.
In the secretory epithelium of the chicken mandibular gland, glycoconjugates have been studied by means of histochemical methods of light and electron microscopy. In light microscopy, a series of histochemical procedures have been employed which included lectin--peroxidase--diaminobenzidine methods and a digestion technique with neuraminidase or alpha-amylase. In electron microscopy, a battery of methods were used that corresponded to those employed in light microscopy. In the secretory cells of the chicken mandibular gland, vicinal diol- and sulphate-containing glycoconjugates with sialic acid, alpha-D-mannose, alpha-D-glucose and beta-D-galactose residues were visualized and the possible histophysiological significances of such glycoconjugates were discussed with special reference to the functions of the salivary gland.
在鸡下颌下腺的分泌上皮中,已通过光学显微镜和电子显微镜的组织化学方法对糖缀合物进行了研究。在光学显微镜下,采用了一系列组织化学程序,包括凝集素-过氧化物酶-二氨基联苯胺法以及用神经氨酸酶或α-淀粉酶的消化技术。在电子显微镜下,使用了一系列与光学显微镜中所采用的方法相对应的方法。在鸡下颌下腺的分泌细胞中,观察到含有邻二醇和硫酸盐的糖缀合物带有唾液酸、α-D-甘露糖、α-D-葡萄糖和β-D-半乳糖残基,并特别参照唾液腺的功能讨论了此类糖缀合物可能的组织生理意义。