Yasui Tadashi, Tsukise Azuma, Habata Isao, Nara Takayuki, Meyer Wilfried
Department of Veterinary Anatomy, College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa, 252-8510 Kanagawa, Japan.
Arch Dermatol Res. 2004 Jun;296(1):12-20. doi: 10.1007/s00403-004-0464-5. Epub 2004 Apr 9.
The localization and chemical nature of complex carbohydrates in the ceruminous glands of the Japanese miniature (Shiba) goat were studied using light and electron microscopic histochemical methods, particularly lectin histochemistry. The epithelial cells and luminal secretion of the caprine ceruminous glands contained large amounts of neutral and smaller amounts of acidic glycoconjugates with different terminal sugars (alpha- d-mannose, alpha-L-fucose, alpha-N-acetyl-D-galactosamine, beta-D-galactose, beta-N-acetyl-D-glucosamine, and N-acetyl-neuraminic acid). Several sugars (alpha-L-fucose, beta-D-galactose, beta-N-acetyl-D-glucosamine, and N-acetyl-neuraminic acid) were also detectable in the secretion of the sebaceous glands. The results obtained are discussed with regard to the specific function of the glandular secretion mixture. The complex glycoconjugates found in the ceruminous gland secretion may control viscoelasticity of and bacterial proliferation within the cerumen in order to protect the external auditory canal against physical damage or microbial attacks.
利用光学和电子显微镜组织化学方法，特别是凝集素组织化学，研究了日本小型（柴犬）山羊耵聍腺中复合碳水化合物的定位和化学性质。山羊耵聍腺的上皮细胞和管腔分泌物含有大量中性糖缀合物和少量酸性糖缀合物，这些糖缀合物具有不同的末端糖（α - D - 甘露糖、α - L - 岩藻糖、α - N - 乙酰 - D - 半乳糖胺、β - D - 半乳糖、β - N - 乙酰 - D - 葡萄糖胺和N - 乙酰神经氨酸）。在皮脂腺分泌物中也可检测到几种糖（α - L - 岩藻糖、β - D - 半乳糖、β - N - 乙酰 - D - 葡萄糖胺和N - 乙酰神经氨酸）。结合腺分泌混合物的特定功能对所得结果进行了讨论。在耵聍腺分泌物中发现的复合糖缀合物可能控制耵聍的粘弹性和细菌增殖，以保护外耳道免受物理损伤或微生物侵袭。
