Stern S J, Flock S T, Small S, Thomsen S, Jacques S
Department of Head and Neck Surgery, University of Texas, M.D. Anderson Cancer Center, Houston 77030.
Am J Surg. 1990 Oct;160(4):360-4. doi: 10.1016/s0002-9610(05)80543-4.
The efficacy of photodynamic therapy tumor destruction is dependent upon both the interruption of the tumor vasculature and the resultant production of unstable oxygen species causing cellular oxidation and death. Chloroaluminum sulfonated phthalocyanine (CASP) is a recently developed photosensitizer. In order to study the direct vascular effects of CASP on a non-tumor system, a rat window chamber was utilized. Twelve rats were implanted with the window chamber, and were divided into two groups of six. Three rats served as controls for each group (receiving light alone, CASP alone, or no treatment). The remaining 6 rats received 10 mg/kg CASP intravenously 4 days after chamber placement. Photoactivation with light was performed 24 hours after injection (power density 200 mW/cm2, irradiance 100 J/cm2, lambda = 675 nm). Utilizing integrating sphere measurements and image analysis, marked vascular changes in the form of initial vasospasm followed by vaso-constriction and loss of chamber neovascularization were noted in the CASP-PDT group. The control groups exhibited no significant changes. Manipulation of the chamber vasculature at strategic time-points may translate into improved response rates for photodynamic therapy in a tumor model.
光动力疗法破坏肿瘤的疗效取决于肿瘤血管的中断以及由此产生的不稳定氧物种导致细胞氧化和死亡。磺化铝酞菁氯（CASP）是一种最近开发的光敏剂。为了研究CASP对非肿瘤系统的直接血管作用，使用了大鼠窗口室。将12只大鼠植入窗口室，并分为两组，每组6只。每组3只大鼠作为对照（分别仅接受光照、仅接受CASP或不进行治疗）。其余6只大鼠在植入窗口室4天后静脉注射10 mg/kg CASP。注射后24小时进行光激活（功率密度200 mW/cm²，辐照度100 J/cm²，波长=675 nm）。利用积分球测量和图像分析，在CASP-光动力疗法组中观察到明显的血管变化，表现为最初的血管痉挛，随后是血管收缩和窗口室新血管形成的丧失。对照组未表现出明显变化。在关键时间点对窗口室血管系统进行操作可能会提高肿瘤模型中光动力疗法的反应率。
