Stern S J, Flock S, Small S, Thomsen S, Jacques S
Department of Otolaryngology-Head and Neck Surgery, Houston, TX 77030.
Otolaryngol Head Neck Surg. 1991 Oct;105(4):556-66. doi: 10.1177/019459989110500407.
In order to study both the anti-tumor effects and early vascular events in photodynamic therapy, a useful animal model has been developed. A window chamber is surgically placed on the dorsum of the Fischer-344 rat, and 500-microns fragments of the rat mammary adenocarcinoma 13672 are placed under direct vision into the subcutaneous tissue. Implantation of the chamber has been successfully completed in more than 50 rats. The operative procedure is straightforward and is accomplished in less than 1 hour. Using tumor fragments, tumor viability has been 60%. We have demonstrated obvious and reproducible neovascularization occurring as soon as 1 day after implantation. The application of this system to an experimental protocol comparing the photosensitizers dihematoporphyrin ether (DHE) and chloraluminum sulfonated phthalocyanine (CASP) has yielded important information on early vascular events resulting from photodynamic therapy.
为了研究光动力疗法中的抗肿瘤作用和早期血管事件，已开发出一种有用的动物模型。通过手术将一个窗口小室放置在Fischer-344大鼠的背部，并在直视下将大鼠乳腺腺癌13672的500微米碎片放置到皮下组织中。已成功地在50多只大鼠身上完成了小室植入。手术操作简单，不到1小时即可完成。使用肿瘤碎片时，肿瘤存活率为60%。我们已经证明，植入后1天就会出现明显且可重复的新生血管形成。将该系统应用于一项比较光敏剂二血卟啉醚（DHE）和磺化铝酞菁（CASP）的实验方案，已获得了有关光动力疗法引起的早期血管事件的重要信息。
