Delvaux A, Lemos M, Moreau C, Erneux C
Institute of Interdisciplinary Research, School of Medicine, Free University of Brussels, Belgium.
Anal Biochem. 1990 Jul;188(1):219-21. doi: 10.1016/0003-2697(90)90555-n.
Regeneration of enzyme activity after sodium dodecyl sulfate-gel electrophoresis was investigated with a purified inositol 1,4,5-trisphosphate 5-phosphatase. In order to avoid silver or Coomassie blue staining, we have used zinc acetate. This staining procedure was sensitive, rapid, and reversible provided that zinc cations are chelated and activity is extracted after diffusion out of the gel. The method allows some gel lane staining and identification of the enzyme based on catalytic activity.
使用纯化的肌醇1,4,5-三磷酸5-磷酸酶研究了十二烷基硫酸钠-凝胶电泳后酶活性的再生情况。为了避免银染或考马斯亮蓝染色，我们使用了醋酸锌。只要锌阳离子被螯合且活性在从凝胶中扩散出来后被提取，这种染色方法就灵敏、快速且可逆。该方法允许对一些凝胶泳道进行染色，并基于催化活性鉴定酶。
