Heukeshoven J, Dernick R
Heinrich-Pette-Institut für Experimentelle Virologie und Immunologie, Universität Hamburg, Federal Republic of Germany.
Electrophoresis. 1988 Jan;9(1):28-32. doi: 10.1002/elps.1150090106.
A new modification of silver staining of proteins in sodium dodecyl sulfate polyacrylamide gels is adapted to automated staining in PhastSystem Development Unit. The use of a reduction step, after fixation, with thiosulfate in alcoholic sodium acetate buffer results in a considerable increase in sensitivity without the need for a recycling step. The detection limit is tenfold lower than in the silver staining procedure recommended so far for PhastSystem and corresponds to 0.05-0.1 ng protein per band. Total staining time with the new procedure is 75 min.
一种用于十二烷基硫酸钠聚丙烯酰胺凝胶中蛋白质银染的新改良方法适用于PhastSystem显影装置中的自动染色。在固定后，使用硫代硫酸盐在乙醇醋酸钠缓冲液中进行还原步骤，可显著提高灵敏度，而无需循环步骤。检测限比目前推荐用于PhastSystem的银染方法低十倍，相当于每条带0.05 - 0.1 ng蛋白质。新方法的总染色时间为75分钟。
