序列选择性药物对弯曲DNA片段凝胶迁移率的影响。

Effects of sequence selective drugs on the gel mobility of a bent DNA fragment.

作者信息

Cons B M, Fox K R

机构信息

Dept. of Physiology & Pharmacology, University of Southampton, U.K.

出版信息

Biochem Biophys Res Commun. 1990 Sep 28;171(3):1064-70. doi: 10.1016/0006-291x(90)90792-l.

DOI:10.1016/0006-291x(90)90792-l

PMID:1699520

Abstract

The effects of various drugs on the structure of a bent DNA fragment have been investigated by studying DNA mobility in polyacrylamide gels. This DNA fragment has an anomalously slow rate of migration on account of its phased runs of adenines. Nogalamycin and echinomycin increase the gel mobility of kinetoplast DNA suggesting that the bending has been removed. Mithramycin, actinomycin, distamycin and ethidium have either no effect or cause a further reduction in mobility. These results are compared with other, non-bent DNA species which always show a decrease in gel mobility in the presence of DNA binding drugs.

摘要

通过研究DNA在聚丙烯酰胺凝胶中的迁移率，已对各种药物对弯曲DNA片段结构的影响进行了研究。该DNA片段由于其腺嘌呤的相位排列而具有异常缓慢的迁移速率。诺加霉素和棘霉素增加了动质体DNA的凝胶迁移率，这表明弯曲已被消除。光神霉素、放线菌素、偏端霉素和溴化乙锭要么没有影响，要么导致迁移率进一步降低。这些结果与其他非弯曲DNA种类进行了比较，在存在DNA结合药物的情况下，这些非弯曲DNA种类的凝胶迁移率总是降低的。