铁通过G蛋白抑制大鼠尾状核中与D-1多巴胺受体偶联的腺苷酸环化酶。

Iron inhibits D-1 dopamine receptor coupled adenylate cyclase via G-proteins in the caudate nucleus of the rat.

作者信息

Moser A, Schuster O

机构信息

Dept. of Neurology, Medical University of Lübeck, F.R.G.

出版信息

Biochem Biophys Res Commun. 1990 Sep 28;171(3):1372-7. doi: 10.1016/0006-291x(90)90837-d.

DOI:10.1016/0006-291x(90)90837-d

PMID:1699528

Abstract

The effects of iron ions (Fe(II)sulfate) on basal, forskolin, and dopamine-stimulated activity of adenylate cyclase in membrane preparations from caudate-putamen of the rat have been studied. Iron dose-dependently inhibited both basal and activated adenylate cyclase activity. In contrast to guanylylimidodiphosphate (Gpp(NH)p), guanosine triphosphate (GTP) was found to enhance this inhibitory effect of iron ions. In addition, cholera toxin was able to antagonize the inhibitory effect of iron on forskolin-activated adenylate cyclase. In our preliminary study we suggest an interaction between iron and the guanine nucleotide regulatory subunit. However, further studies are necessary.

摘要

研究了铁离子（硫酸亚铁）对大鼠尾状核 - 壳核膜制剂中基础、福斯可林及多巴胺刺激的腺苷酸环化酶活性的影响。铁离子剂量依赖性地抑制基础和激活的腺苷酸环化酶活性。与鸟苷酰亚胺二磷酸（Gpp(NH)p）相反，发现三磷酸鸟苷（GTP）可增强铁离子的这种抑制作用。此外，霍乱毒素能够拮抗铁对福斯可林激活的腺苷酸环化酶的抑制作用。在我们的初步研究中，我们提出铁与鸟嘌呤核苷酸调节亚基之间存在相互作用。然而，还需要进一步研究。