氧化高铁骨化醇对大鼠纹状体腺苷酸环化酶的作用。

The effect of oxiferriscorbone on striatal adenylate cyclase of the rat.

作者信息

Moser A, Schuster O

机构信息

Department of Neurology, Medical University of Lübeck, F.R.G.

出版信息

Neuropharmacology. 1990 Aug;29(8):731-4. doi: 10.1016/0028-3908(90)90126-c.

DOI:10.1016/0028-3908(90)90126-c

PMID:2274108

Abstract

The effects of oxiferriscorbone on basal and forskolin-stimulated activity of adenylate cyclase in membrane preparations from caudate-putamen of the rat have been studied. Oxiferriscorbone, at 30 microM, stimulated the basal activity of the enzyme, but dose-dependently inhibited forskolin-activated activity of adenylate cyclase. Pertussis toxin was found to antagonize this inhibitory effect of oxiferriscorbone. Dopamine stimulated the activity of adenylate cyclase in the striatum, as described previously. When assayed together, the stimulating effects of dopamine and oxiferriscorbone were were additive, implying that they do not act at identical sites. The D1 receptor antagonist, alpha (+)-flupentixol completely blocked the effect of dopamine but had no significant effect on oxiferriscorbone-induced stimulation. The present results suggest that interaction between oxiferriscorbone and the inhibitory guanine nucleotide subunit. However, further studies are necessary.

摘要

研究了氧化苦参碱对大鼠尾状核-壳核膜制剂中腺苷酸环化酶基础活性和福斯高林刺激活性的影响。30微摩尔的氧化苦参碱刺激了该酶的基础活性，但剂量依赖性地抑制了福斯高林激活的腺苷酸环化酶活性。发现百日咳毒素可拮抗氧化苦参碱的这种抑制作用。如先前所述，多巴胺刺激纹状体中腺苷酸环化酶的活性。当一起测定时，多巴胺和氧化苦参碱的刺激作用是相加的，这意味着它们作用于不同的位点。D1受体拮抗剂α(+)-氟哌噻吨完全阻断了多巴胺的作用，但对氧化苦参碱诱导的刺激没有显著影响。目前的结果表明氧化苦参碱与抑制性鸟嘌呤核苷酸亚基之间存在相互作用。然而，还需要进一步的研究。