Marannino Pierdomenico, Santiago-Alvarez Cándido, de Lillo Enrico, Quesada-Moraga Enrique
Departamento de Ciencias y Recursos Agrícolas y Forestales, ETSIAM, Universidad de Córdoba, Campus de Rabanales, Edificio C4 Celestino Mutis, Cordoba 14071, Spain.
J Invertebr Pathol. 2006 Nov;93(3):210-3. doi: 10.1016/j.jip.2006.08.002. Epub 2006 Sep 22.
We used a newly developed bioassay method to demonstrate for the first time the potential of the entomopathogenic fungi Beauveria bassiana and Metarhizium anisopliae to be used for the control of neonate larvae of Capnodis tenebrionis, a major threat to stone-fruit orchards in several countries. Four B. bassiana and four M. anisopliae isolates were all pathogenic for neonate larvae of C. tenebrionis; mortality rates 10 days after inoculation by dipping in a suspension with 10(8)conidia/ml varied from 23.5% to 100%. Three of the four M. anisopliae isolates caused 100% mortality. In most cases, postmortem hyphal growth and sporulation of M. anisopliae or B. bassiana was observed covering the larvae in their galleries. The eight isolates were also evaluated for pathogenicity to C. tenebrionis eggs at the same dosage. Only two B. bassiana isolates caused significant egg hatching reduction of 84.5% and 94.5%. Our results indicate that M. anisopliae and B. bassiana may be considered as promising for a new approach to prevent larval infestations by C. tenebrionis.
我们采用一种新开发的生物测定方法，首次证明了昆虫病原真菌球孢白僵菌和绿僵菌用于防治坚果黑条象甲初孵幼虫的潜力，坚果黑条象甲是多个国家核果类果园的主要威胁。四种球孢白僵菌和四种绿僵菌分离株对坚果黑条象甲初孵幼虫均具有致病性；通过浸蘸于含10⁸ 个分生孢子/毫升的悬浮液中接种10天后的死亡率在23.5%至100%之间。四种绿僵菌分离株中的三种导致了100%的死亡率。在大多数情况下，观察到绿僵菌或球孢白僵菌在幼虫虫道内的尸体上生长并形成孢子。以相同剂量对这八种分离株对坚果黑条象甲卵的致病性也进行了评估。只有两种球孢白僵菌分离株导致显著的卵孵化率降低，分别为84.5%和94.5%。我们的结果表明，绿僵菌和球孢白僵菌有望成为防治坚果黑条象甲幼虫侵害的新方法。
