Departamento de Produção Vegetal, Faculdade de Ciências Agrárias e Veterinárias (FCAV), Universidade Estadual Paulista (Unesp), Via de Acesso Prof. Paulo Donato Castellane, s/no, CEP 14884-900, Jaboticabal, SP, Brazil.
Vet Parasitol. 2010 Jan 20;167(1):62-6. doi: 10.1016/j.vetpar.2009.09.046. Epub 2009 Oct 9.
The present study assessed the pathogenic effect of isolates E9, IBCB425 and IBCB159 of the Metarhizium anisopliae fungus, JAB06, JAB07 and AM09 of Beauveria bassiana, IBCB133 and CB75 of Isaria fumosorosea (=Paecilomyces fumosoroseus) and CG189 and CG195 of Isaria farinosa (=Paecilomyces farinosus) against eggs and larvae of the horn fly Haematobia irritans. Eggs were inoculated with suspensions containing 10(6), 10(7) and 10(8)conidiaml(-1) of the fungal isolates and observed after 48h to determine viability. In the larvae study, eggs were allowed to hatch into fresh bovine feces that had been treated with 10(8), 10(7) or 10(6)conidiamgfeces(-1). In both studies, 5 days after initial procedures, all formed pupae were transferred to an incubator at 27+/-0.5 degrees C until the emergence of the adult flies was complete. The M. anisopliae isolates did not cause the death of H. irritans eggs, but they did promote the death of larvae that hatched from treated eggs, and therefore increased the total mortality. Isolate E9 promoted 100% mortality of treated larvae at a concentration of 10(8)conidiaml(-1). For the B. bassiana isolates, no activity was observed against insect eggs or larvae. Both I. fumosorosea isolates promoted significant mortality (p<0.05) of eggs at every concentration of conidia. Isolate CG195 of I. farinosa increased the mortality of larvae and pupae that hatched from treated eggs and promoted significant total mortality (p<0.05) of the insect at every concentration of conidia.
本研究评估了绿僵菌（Metarhizium anisopliae）菌株 E9、IBCB425 和 IBCB159、球孢白僵菌（Beauveria bassiana）菌株 JAB06、JAB07 和 AM09、玫烟色棒束孢（Isaria fumosorosea）=拟青霉（Paecilomyces fumosoroseus）菌株 IBCB133 和 CB75 以及深绿木霉（Isaria farinosa）=拟青霉（Paecilomyces farinosus）菌株 CG189 和 CG195 对牛角蝇（Haematobia irritans）卵和幼虫的致病性。将含有 10(6)、10(7)和 10(8)cfu·mL(-1)的真菌悬浮液接种到卵中，48 小时后观察其活力。在幼虫研究中，将卵孵化到已用 10(8)、10(7)或 10(6)cfu·g(-1)粪便处理过的新鲜牛粪便中。在这两项研究中，初始处理后 5 天，所有形成的蛹都转移到 27+/-0.5°C 的孵育箱中，直到成虫完全出现。绿僵菌分离物不会导致牛角蝇卵死亡，但它们确实促进了来自处理过的卵孵化的幼虫死亡，从而增加了总死亡率。在 10(8)cfu·mL(-1)浓度下，分离物 E9 可促进 100%的处理幼虫死亡。对于白僵菌分离物，对昆虫卵或幼虫没有活性。两种玫烟色棒束孢分离物在每个孢子浓度下都显著促进了卵的死亡率（p<0.05）。深绿木霉（Isaria farinosa）分离物 CG195 增加了来自处理过的卵孵化的幼虫和蛹的死亡率，并在每个孢子浓度下显著促进了昆虫的总死亡率（p<0.05）。
