Kim Hyunsoo, Kim Ick Young, Lee Soo Young, Jeong Daewon
Division of Molecular Life Sciences and the Center for Cell Signaling Research, Ewha Womans University, Room# 310, Science Building C, Seodaemun-gu Daehyun-dong 11-1, Seoul 120-750, Republic of Korea.
FEBS Lett. 2006 Oct 16;580(24):5661-5. doi: 10.1016/j.febslet.2006.09.015. Epub 2006 Sep 18.
In order to demonstrate that cellular redox status undergoes decreased reduction during osteoclast differentiation and further decreased reduction during osteoclastic bone resorption, we analyzed gamma-glutamylcysteinyl synthetase activity, a glutathione synthesis rate-limiting enzyme, and total glutathione and thiol groups. Moderate and severe redox shifts towards a more oxidizing environment induced gradual increases and decreases in osteoclastogenesis. Moreover, while severe glutathione depletion inhibited bone resorption, moderate glutathione repletion enhanced bone resorption. In summary, our observations suggest that there is a threshold for redox status, representing biphasic patterns in osteoclast differentiation and function.
为了证明破骨细胞分化过程中细胞氧化还原状态的还原程度降低，以及在破骨细胞骨吸收过程中还原程度进一步降低，我们分析了γ-谷氨酰半胱氨酸合成酶活性（一种谷胱甘肽合成限速酶）、总谷胱甘肽和硫醇基团。向更具氧化性环境的中度和重度氧化还原转变导致破骨细胞生成逐渐增加和减少。此外，虽然严重的谷胱甘肽耗竭会抑制骨吸收，但适度的谷胱甘肽补充会增强骨吸收。总之，我们的观察结果表明，氧化还原状态存在一个阈值，在破骨细胞分化和功能中呈现双相模式。
