Kamon M, Fujita D, Goto N, Amano H, Sakamoto K
Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki, Japan.
Prostaglandins Other Lipid Mediat. 2008 Dec;87(1-4):26-33. doi: 10.1016/j.prostaglandins.2008.06.002. Epub 2008 Jun 27.
Prostaglandin (PG) E(2) promotes osteoclastic cell differentiation, but the physiological function of PGF(2alpha) remains unclear. We examined the physiological effects of PGF(2alpha) on osteoclast differentiation using a murine cell line, RAW264, and the column-purified murine bone marrow cells, both of which are differentiable into osteoclast-like multi-nuclear cells. Although PGF(2alpha) did not affect the number of differentiated osteoclasts, PGF(2alpha) reduced the bone resorption activity of osteoclasts developed from both cell types in a pit formation assay. Thus, PGF(2alpha) inhibits bone resorption without affecting the number of osteoclasts, providing a novel molecular mechanism underlying bone metabolism.
前列腺素（PG）E2促进破骨细胞分化，但前列腺素F2α（PGF2α）的生理功能仍不清楚。我们使用小鼠细胞系RAW264和经柱纯化的小鼠骨髓细胞研究了PGF2α对破骨细胞分化的生理作用，这两种细胞均可分化为破骨细胞样多核细胞。虽然PGF2α不影响分化的破骨细胞数量，但在小坑形成试验中，PGF2α降低了由这两种细胞类型发育而来的破骨细胞的骨吸收活性。因此，PGF2α在不影响破骨细胞数量的情况下抑制骨吸收，为骨代谢提供了一种新的分子机制。
