Garrido Nicolás, Remohí José, Pellicer Antonio, Meseguer Marcos
Instituto Universitario IVI, Valencia, Spain.
Fertil Steril. 2006 Nov;86(5):1544-6. doi: 10.1016/j.fertnstert.2006.03.061. Epub 2006 Sep 25.
Men who are seropositive for human immunodeficiency virus (HIV) or hepatitis C virus now can father children safely, thanks to sperm washing and nested polymerase chain-reaction techniques, followed by intracytoplasmic sperm injection. However, in a small percentage of such patients, it is impossible to recover spermatozoa after said procedures because of their highly impaired spermatogenesis. We have established that less rigorous methods, such as repeated centrifugation, yield nested polymerase chain-reaction HIV- and hepatitis C virus-negative specimens, even in sperm samples from men with severe oligoasthenozoospermia.
由于精子洗涤和巢式聚合酶链反应技术，随后进行胞浆内单精子注射，感染人类免疫缺陷病毒（HIV）或丙型肝炎病毒且血清学呈阳性的男性现在可以安全地生育子女。然而，在一小部分此类患者中，由于精子发生严重受损，在上述操作后无法获得精子。我们已经确定，即使是来自严重少弱精子症男性的精子样本，采用不太严格的方法，如反复离心，也能得到巢式聚合酶链反应检测HIV和丙型肝炎病毒呈阴性的样本。
