Nilson Theresa L, Sinclair Brent J, Roberts Stephen P
Department of Biological Sciences University of Nevada, Las Vegas, NV 89154-4004, USA.
J Insect Physiol. 2006 Oct;52(10):1027-33. doi: 10.1016/j.jinsphys.2006.07.001. Epub 2006 Jul 27.
Carbon dioxide gas is used as an insect anesthetic in many laboratories, despite recent studies which have shown that CO(2) can alter behavior and fitness. We examine the effects of CO(2) and anoxia (N(2)) on cold tolerance, measuring the rapid cold-hardening (RCH) response and chill coma recovery in Drosophila melanogaster. Short exposures to CO(2) or N(2) do not significantly affect RCH, but 60 min of exposure negates RCH. Exposure to CO(2) anesthesia increases chill coma recovery time, but this effect disappears if the flies are given 90 min recovery in air before chill coma induction. Flies treated with N(2) show a similar pattern, but require significantly longer chill coma recovery times even after 90 min of recovery from anoxia. Our results suggest that CO(2) anesthesia is an acceptable way to manipulate flies before cold tolerance experiments (when using RCH or chill coma recovery as a measure), provided exposure duration is minimized and recovery is permitted before chill coma induction. However, we recommend that exposure to N(2) not be used as a method of anesthesia for chill coma studies.
尽管最近的研究表明二氧化碳（CO₂）会改变行为和健康状况，但在许多实验室中，二氧化碳气体仍被用作昆虫麻醉剂。我们研究了CO₂和缺氧（N₂）对耐寒性的影响，测量了黑腹果蝇的快速冷驯化（RCH）反应和冷昏迷恢复情况。短时间暴露于CO₂或N₂对RCH没有显著影响，但暴露60分钟会使RCH消失。暴露于CO₂麻醉会增加冷昏迷恢复时间，但如果在诱导冷昏迷前让果蝇在空气中恢复90分钟，这种影响就会消失。用N₂处理的果蝇表现出类似的模式，但即使在缺氧恢复90分钟后，它们的冷昏迷恢复时间也明显更长。我们的结果表明，在耐寒性实验之前（当使用RCH或冷昏迷恢复作为衡量标准时），CO₂麻醉是一种可接受的处理果蝇的方法，前提是将暴露时间减至最短，并在诱导冷昏迷前允许恢复。然而，我们建议不要将暴露于N₂用作冷昏迷研究的麻醉方法。
