Burr D B, Stafford T
Department of Anatomy, West Virginia University Health Sciences Center, Morgantown.
Clin Orthop Relat Res. 1990 Nov(260):305-8.
Frost's bulk-staining technique clearly separates artifactual cracking in bone from that caused by mechanical loading in vivo. One-centimeter sections of human rib were either bulk stained before preparation of a thin section (experimental) or stained after grinding to a thickness of 150 microns (control). Microdamage produced before preparation can be separated from that caused by the preparation itself. Moreover, the technique does not cause additional artifactual cracking through dehydration. This technique provides a valid way to test hypotheses about the role that microdamage creation or its repair plays in skeletal pathology.
弗罗斯特的整体染色技术能够清晰地区分骨骼中的人为裂缝与体内机械负荷导致的裂缝。将一厘米长的人类肋骨切片，要么在制备薄片之前进行整体染色（实验组），要么在研磨至150微米厚度后进行染色（对照组）。制备前产生的微损伤可以与制备过程本身造成的微损伤区分开来。此外，该技术不会因脱水而导致额外的人为裂缝。这项技术为检验关于微损伤的产生或其修复在骨骼病理学中所起作用的假设提供了一种有效的方法。
