Klein C P, Sauren Y M, Modderman W E, van der Waerden J P
Department of Biomaterials, School of Medicine, University of Leiden, The Netherlands.
J Appl Biomater. 1994 Winter;5(4):369-73. doi: 10.1002/jab.770050413.
A newly developed saw technique was developed to produce sections circa 10 microns or thicker from fresh bone or dentine and from plastic embedded undecalcified bone tissue with or without implant materials. The method comprises only one step because grinding or polishing to make the sections thinner is not necessary. The bone slices can be decalcified rapidly without using aggressive solvents and used for making ultrathin sections for electron microscopy. Sections of fresh dentine of 15 to 30 microns are transparent which makes it possible to study osteoclastic resorption in vitro. Sections, 10 microns thick, with an intact interface of bone and implant material can be observed for biocompatibility studies.
一种新开发的锯切技术被用于从新鲜骨骼或牙本质以及含有或不含有植入材料的塑料包埋未脱钙骨组织中制备约10微米或更厚的切片。该方法仅包含一个步骤，因为无需研磨或抛光以使切片更薄。骨切片可以在不使用侵蚀性溶剂的情况下快速脱钙，并用于制作电子显微镜用的超薄切片。15至30微米厚的新鲜牙本质切片是透明的，这使得在体外研究破骨细胞吸收成为可能。对于生物相容性研究，可以观察到厚度为10微米且骨与植入材料界面完整的切片。
