He Xuan, Lu Lidan, Huang Ping, Yu Bo, Peng Lianxin, Zou Liang, Ren Yuanhang
Key Laboratory of Coarse Cereal Processing, Ministry of Agriculture and Rural Affairs, Sichuan Engineering and Technology Research Center of Coarse Cereal Industralization, College of Food and Biological Engineering, Chengdu University, Chengdu 610106, China.
Insects. 2023 Jan 18;14(2):104. doi: 10.3390/insects14020104.
During the past decades, research on insect cell culture has grown tremendously. Thousands of lines have been established from different species of insect orders, originating from several tissue sources. These cell lines have often been employed in insect science research. In particular, they have played important roles in pest management, where they have been used as tools to evaluate the activity and explore the toxic mechanisms of insecticide candidate compounds. This review intends to first briefly summarize the progression of insect cell line establishment. Then, several recent studies based on insect cell lines coupled with advanced technologies are introduced. These investigations revealed that insect cell lines can be exploited as novel models with unique advantages such as increased efficiency and reduced cost compared with traditional insecticide research. Most notably, the insect cell line-based models provide a global and in-depth perspective to study the toxicology mechanisms of insecticides. However, challenges and limitations still exist, especially in the connection between in vitro activity and in vivo effectiveness. Despite all this, recent advances have suggested that insect cell line-based models promote the progress and sensible application of insecticides, which benefits pest management.
在过去几十年中，昆虫细胞培养研究取得了巨大进展。已从昆虫纲的不同物种中建立了数千个细胞系，这些细胞系源自多种组织来源。这些细胞系经常被用于昆虫科学研究。特别是，它们在害虫管理中发挥了重要作用，被用作评估杀虫剂候选化合物活性和探索其毒理机制的工具。本综述旨在首先简要总结昆虫细胞系建立的进展。然后，介绍了几项基于昆虫细胞系并结合先进技术的最新研究。这些研究表明，与传统杀虫剂研究相比，昆虫细胞系可作为具有独特优势的新型模型，如提高效率和降低成本。最值得注意的是，基于昆虫细胞系的模型为研究杀虫剂的毒理学机制提供了全面而深入的视角。然而，挑战和局限性仍然存在，尤其是在体外活性与体内有效性之间的联系方面。尽管如此，最近的进展表明，基于昆虫细胞系的模型促进了杀虫剂的发展和合理应用，这有利于害虫管理。
