Inceoglu A Bora, Kamita S George, Hammock Bruce D
Department of Entomology and Cancer Research Center University of California, Davis, California 95616, USA.
Adv Virus Res. 2006;68:323-60. doi: 10.1016/S0065-3527(06)68009-3.
The concept of using genetic engineering to improve the natural insecticidal activity of baculoviruses emerged during the 1980s. Both academic and industrial laboratories have since invested a great deal of effort to generate genetically modified (GM) or recombinant baculoviruses with dramatically improved speeds of kill. Optimal production methodologies and formulations have also been developed, and the safety and ecology of the recombinant baculoviruses have been thoroughly investigated. Unfortunately, the initial excitement that was generated by these technologies was tempered when industry made a critical decision to not complete the registration process of GM baculoviruses for pest insect control. In this chapter, we summarize the developments in the field from a historical perspective and provide our opinions as to the current status and future potential of the technology. We will argue that GM baculoviruses are valuable and viable tools for pest insect control both alone and in combination with wild-type viruses. We believe that these highly effective biopesticides still have a bright future in modern agriculture as public awareness and acceptance of GM organisms, including GM baculoviruses, increases.
利用基因工程提高杆状病毒天然杀虫活性的概念始于20世纪80年代。从那时起，学术和工业实验室都投入了大量精力来培育具有显著提高杀灭速度的转基因（GM）或重组杆状病毒。还开发了最佳生产方法和制剂，并对重组杆状病毒的安全性和生态学进行了深入研究。不幸的是，当行业做出不完成用于害虫防治的转基因杆状病毒注册程序的关键决定时，这些技术最初带来的兴奋情绪有所缓和。在本章中，我们从历史角度总结了该领域的发展，并就该技术的现状和未来潜力发表我们的看法。我们将论证，转基因杆状病毒无论是单独使用还是与野生型病毒联合使用，都是用于害虫防治的有价值且可行的工具。我们相信，随着公众对包括转基因杆状病毒在内转基因生物的认识和接受度提高，这些高效生物杀虫剂在现代农业中仍有光明的未来。
