Tanito Masaki, Kaidzu Sachiko, Anderson Robert E
Department of Ophthalmology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA.
Exp Eye Res. 2006 Dec;83(6):1493-504. doi: 10.1016/j.exer.2006.08.006. Epub 2006 Sep 25.
Recently, a yellow intraocular lens (IOL) was developed for the purpose of reducing potential blue light-induced retinal damage after cataract surgery. However, the effect of yellow filters on retinal protection remains to be clarified. To test the protective effects of yellow filters on blue light-induced retinal damage, a yellow and a clear soft acrylic filter were attached to the right and left eyes, respectively, of albino rats and exposed to 4.5 k lux blue fluorescent lights with peak wavelength at 420 nm (ranging 380-500 nm; short blue) or 446 nm (ranging 400-540 nm; long blue) for 6h. To assess retinal damage, the electroretinogram (ERG) was recorded at 7 days, outer nuclear layer (ONL) thickness and area were measured at 7 days, apoptosis was analyzed by TUNEL staining at 24 h, and the level of lipid peroxidation in retinas was assessed by Western dot blots using specific antibodies against 4-hydroxynonenal (4-HNE)- and carboxyethylpyrrole (CEP)-modified proteins immediately after light exposure. After short blue light exposure, a- and b-wave ERG amplitudes and the ONL thickness at 1-2.5 mm inferior and 0.5-2.5 mm superior to optic nerve head (ONH) were significantly reduced. TUNEL staining in the ONL at 0-2 mm inferior and 1-2 mm superior to the ONH, and retinal levels of 4-HNE- and CEP-modified proteins were significantly increased in the clear filter-covered eyes compared to yellow filter-covered eyes. After long blue light exposure, the only difference seen was a greater ONL thickness at 1.5 mm superior to the ONH in yellow filter-covered eye. Transmission of light through the yellow filter was 58% for short blue and 89% for long blue compared to the clear filter. The ONL area was not different between clear filter-covered and -uncovered eyes after exposure to short or long blue light. Given the results, yellow IOL material protects the retina against acute shorter wavelength blue light exposure more effectively than the clear IOL material.
最近，为了减少白内障手术后潜在的蓝光诱导的视网膜损伤，一种黄色人工晶状体（IOL）被研发出来。然而，黄色滤光片对视网膜的保护作用仍有待阐明。为了测试黄色滤光片对蓝光诱导的视网膜损伤的保护作用，分别将一片黄色和一片透明的软质丙烯酸滤光片贴在白化大鼠的右眼和左眼中，并使其暴露于峰值波长为420nm（波长范围380 - 500nm；短波蓝光）或446nm（波长范围400 - 540nm；长波蓝光）的4.5klux蓝色荧光灯下6小时。为了评估视网膜损伤，在第7天记录视网膜电图（ERG），在第7天测量外核层（ONL）厚度和面积，在24小时通过TUNEL染色分析细胞凋亡，并在光照后立即使用针对4 - 羟基壬烯醛（4 - HNE）和羧乙基吡咯（CEP）修饰蛋白的特异性抗体通过蛋白质免疫印迹法评估视网膜中的脂质过氧化水平。短波蓝光照射后，距视神经乳头（ONH）下方1 - 2.5mm和上方0.5 - 2.5mm处的a波和b波ERG振幅以及ONL厚度显著降低。与黄色滤光片覆盖的眼睛相比，透明滤光片覆盖的眼睛在距ONH下方0 - 2mm和上方1 - 2mm处的ONL中TUNEL染色以及视网膜中4 - HNE和CEP修饰蛋白的水平显著升高。长波蓝光照射后，唯一的差异是黄色滤光片覆盖的眼睛在距ONH上方1.5mm处的ONL厚度更大。与透明滤光片相比，黄色滤光片对短波蓝光的透光率为58%，对长波蓝光的透光率为89%。在暴露于短波或长波蓝光后，透明滤光片覆盖和未覆盖的眼睛之间的ONL面积没有差异。基于这些结果，黄色IOL材料比透明IOL材料更有效地保护视网膜免受急性较短波长蓝光的照射。
