Yamaguchi Y, Matsui T, Kasahara T, Etoh S, Tominaga A, Takatsu K, Miura Y, Suda T
Department of Medical Biology and Parasitology, Jichi Medical School, Japan.
Exp Hematol. 1990 Dec;18(11):1152-7.
It has been demonstrated that purified recombinant interleukin 5 (rIL-5) supports the terminal differentiation and proliferation of eosinophilic precursors in vitro and plays an important role in increasing the functional activities of eosinophils. In this study, we examined the hemopoietic changes and analyzed murine (m) IL-5 mRNA expression in eosinophilic mice infected with the helminth Toxocara canis. In eosinophilic mice, eosinophils increased in number in both bone marrow and spleen. However, the number of eosinophilic precursors increased markedly in spleen cells of eosinophilic mice but remained relatively constant in the bone marrow. In the presence of granulocyte colony-stimulating factor (G-CSF), the number of granulocytic precursors increased in the spleen cells of eosinophilic mice. From these findings, the condition of eosinophilopoiesis in eosinophilic mice is accompanied by an increase in granulocyte-macrophage progenitors as well as eosinophil progenitors. Using Northern blot analysis, a weak but definite band corresponding to mIL-5 mRNA was detected in spleen cells of mice 4 and 5 days after helminthic infection. In addition, these data were confirmed by in vitro polymerase chain reaction (PCR) amplification of mRNA obtained from these spleen cells. Finally, injections of a monoclonal antibody against mIL-5 completely suppressed the blood eosinophilia in mice infected with T. canis. In conclusion, IL-5 is suggested to play a major role in eosinophilopoiesis in vivo.
已证实，纯化的重组白细胞介素5（rIL-5）在体外支持嗜酸性粒细胞前体的终末分化和增殖，并在增强嗜酸性粒细胞的功能活性方面发挥重要作用。在本研究中，我们检查了感染犬弓首蛔虫的嗜酸性粒细胞增多小鼠的造血变化，并分析了小鼠（m）IL-5 mRNA的表达。在嗜酸性粒细胞增多小鼠中，骨髓和脾脏中的嗜酸性粒细胞数量均增加。然而，嗜酸性粒细胞增多小鼠脾细胞中的嗜酸性粒细胞前体数量显著增加，而骨髓中的数量相对保持恒定。在存在粒细胞集落刺激因子（G-CSF）的情况下，嗜酸性粒细胞增多小鼠脾细胞中的粒细胞前体数量增加。从这些发现来看，嗜酸性粒细胞增多小鼠的嗜酸性粒细胞生成状况伴随着粒细胞-巨噬细胞祖细胞以及嗜酸性粒细胞祖细胞的增加。使用Northern印迹分析，在蠕虫感染后4天和5天的小鼠脾细胞中检测到一条与mIL-5 mRNA相对应的微弱但明确的条带。此外，这些数据通过对从这些脾细胞获得的mRNA进行体外聚合酶链反应（PCR）扩增得到了证实。最后，注射抗mIL-5单克隆抗体完全抑制了感染犬弓首蛔虫小鼠的血液嗜酸性粒细胞增多。总之，提示IL-5在体内嗜酸性粒细胞生成中起主要作用。
