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小鼠骨髓中嗜酸性粒细胞谱系定向祖细胞的鉴定。

Identification of eosinophil lineage-committed progenitors in the murine bone marrow.

作者信息

Iwasaki Hiromi, Mizuno Shin-ichi, Mayfield Robin, Shigematsu Hirokazu, Arinobu Yojiro, Seed Brian, Gurish Michael F, Takatsu Kiyoshi, Akashi Koichi

机构信息

Department of Cancer Immunology and AIDS, Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA 02115, USA.

出版信息

J Exp Med. 2005 Jun 20;201(12):1891-7. doi: 10.1084/jem.20050548. Epub 2005 Jun 13.

DOI:10.1084/jem.20050548
PMID:15955840
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2212039/
Abstract

Eosinophil lineage-committed progenitors (EoPs) are phenotypically isolatable in the steady-state murine bone marrow. Purified granulocyte/monocyte progenitors (GMPs) gave rise to eosinophils as well as neutrophils and monocytes at the single cell level. Within the short-term culture of GMPs, the eosinophil potential was found exclusively in cells activating the transgenic reporter for GATA-1, a transcription factor capable of instructing eosinophil lineage commitment. These GATA-1-activating cells possessed an IL-5Ralpha(+)CD34(+)c-Kit(lo) phenotype. Normal bone marrow cells also contained IL-5Ralpha(+)CD34(+)c-Kit(lo) EoPs that gave rise exclusively to eosinophils. EoPs significantly increased in number in response to helminth infection, suggesting that the EoP stage is physiologically involved in eosinophil production in vivo. EoPs expressed eosinophil-related genes, such as the eosinophil peroxidase and the major basic protein, but did not express basophil/mast cell-related mast cell proteases. The enforced retroviral expression of IL-5Ralpha in GMPs did not enhance the frequency of eosinophil lineage read-outs, whereas IL-5Ralpha(+) GMPs displayed normal neutrophil/monocyte differentiation in the presence of IL-5 alone. Thus, IL-5Ralpha might be expressed specifically at the EoP stage as a result of commitment into the eosinophil lineage. The newly identified EoPs could be the cellular target in the treatment of a variety of disorders mediated by eosinophils.

摘要

嗜酸性粒细胞谱系定向祖细胞（EoP）在稳态小鼠骨髓中可通过表型分离得到。纯化的粒细胞/单核细胞祖细胞（GMP）在单细胞水平上可产生嗜酸性粒细胞以及中性粒细胞和单核细胞。在GMP的短期培养中，嗜酸性粒细胞分化潜能仅存在于激活GATA-1转基因报告基因的细胞中，GATA-1是一种能够指导嗜酸性粒细胞谱系定向分化的转录因子。这些激活GATA-1的细胞具有IL-5Rα(+)CD34(+)c-Kit(lo)表型。正常骨髓细胞中也含有IL-5Rα(+)CD34(+)c-Kit(lo) EoP，其仅产生嗜酸性粒细胞。蠕虫感染后，EoP数量显著增加，提示EoP阶段在体内嗜酸性粒细胞生成过程中发挥生理作用。EoP表达嗜酸性粒细胞相关基因，如嗜酸性粒细胞过氧化物酶和主要碱性蛋白，但不表达嗜碱性粒细胞/肥大细胞相关的肥大细胞蛋白酶。在GMP中强制逆转录病毒表达IL-5Rα并不会增加嗜酸性粒细胞谱系分化的频率，而IL-5Rα(+) GMP在仅存在IL-5的情况下显示出正常的中性粒细胞/单核细胞分化。因此，IL-5Rα可能是由于定向分化为嗜酸性粒细胞谱系而在EoP阶段特异性表达。新鉴定出的EoP可能是治疗多种嗜酸性粒细胞介导疾病的细胞靶点。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aef3/2212039/81f21358a966/20050548f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aef3/2212039/eeb205cfccaf/20050548f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aef3/2212039/db2c9bc94130/20050548f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aef3/2212039/c41c062fef99/20050548f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aef3/2212039/81f21358a966/20050548f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aef3/2212039/eeb205cfccaf/20050548f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aef3/2212039/db2c9bc94130/20050548f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aef3/2212039/c41c062fef99/20050548f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aef3/2212039/81f21358a966/20050548f4.jpg

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J Allergy Clin Immunol. 2004 Jan;113(1):115-9. doi: 10.1016/j.jaci.2003.10.049. Epub 2003 Dec 12.
2
GATA-1 converts lymphoid and myelomonocytic progenitors into the megakaryocyte/erythrocyte lineages.GATA-1将淋巴样和髓单核细胞祖细胞转化为巨核细胞/红细胞谱系。
Immunity. 2003 Sep;19(3):451-62. doi: 10.1016/s1074-7613(03)00242-5.
3
Transcription factor-mediated lineage switching reveals plasticity in primary committed progenitor cells.
Cell Mol Gastroenterol Hepatol. 2025;19(2):101413. doi: 10.1016/j.jcmgh.2024.101413. Epub 2024 Sep 28.
4
Stress-induced eosinophil activation contributes to postoperative morbidity and mortality after lung resection.应激诱导的嗜酸性粒细胞活化导致肺切除术后发病率和死亡率增加。
Sci Transl Med. 2024 Aug 21;16(761):eadl4222. doi: 10.1126/scitranslmed.adl4222.
5
Insight into IL-5 as a Potential Target for the Treatment of Allergic Diseases.深入了解白细胞介素-5作为治疗过敏性疾病的潜在靶点。
Biomedicines. 2024 Jul 10;12(7):1531. doi: 10.3390/biomedicines12071531.
6
Spatial adaptation of eosinophils and their emerging roles in homeostasis, infection and disease.嗜酸性粒细胞的空间适应性及其在稳态、感染和疾病中的新兴作用。
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7
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