Erden Omer, Imir Ayse, Guvenal Tevfik, Muslehiddinoglu Ahmet, Arici Sema, Cetin Meral, Cetin Ali
Department of Obstetrics and Gynaecology, Cumhuriyet University School of Medicine, Sivas, Turkey.
Hum Reprod. 2006 Nov;21(11):3014-8. doi: 10.1093/humrep/del262. Epub 2006 Sep 22.
Heparin and low molecular weight heparin (LMWH) are used widely to improve the pregnancy outcome in women with thrombophilia, miscarriage, recurrent miscarriage and fetal death. This study was designed to investigate the effects of heparin and LMWHs, enoxaparin and tinzaparin, on E-cadherin and laminin expression in placental and decidual tissues in rat pregnancy.
Wistar albino female rats (n = 48) were randomly assigned to four study groups (normal saline, heparin, enoxaparin and tinzaparin) in the preconceptional period. Tissue sections of placenta and decidua were immunohistochemically examined for the expression of E-cadherin and laminin.
E-cadherin placental staining score of heparin group was significantly lower and E-cadherin decidual staining score of heparin and enoxaparin groups were significantly lower than control group. There were no significant differences in placental and decidual laminin staining scores among the study groups.
Heparin and enoxaparin can reduce E-cadherin expression but not laminin expression in rat pregnancy. They might modulate trophoblast invasion. We suggest that this is the possible underlying mechanism involving in improvement of trophoblast invasion by the use of heparin and LMWH in patients with the history of miscarriage.
肝素和低分子量肝素（LMWH）被广泛用于改善患有血栓形成倾向、流产、复发性流产和胎儿死亡的女性的妊娠结局。本研究旨在探讨肝素和低分子量肝素（依诺肝素和替扎肝素）对大鼠妊娠胎盘和蜕膜组织中E-钙黏蛋白和层粘连蛋白表达的影响。
将48只Wistar白化雌性大鼠在孕前随机分为四个研究组（生理盐水组、肝素组、依诺肝素组和替扎肝素组）。对胎盘和蜕膜的组织切片进行免疫组织化学检查，以检测E-钙黏蛋白和层粘连蛋白的表达。
肝素组的胎盘E-钙黏蛋白染色评分显著低于对照组，肝素组和依诺肝素组的蜕膜E-钙黏蛋白染色评分显著低于对照组。各研究组胎盘和蜕膜层粘连蛋白染色评分无显著差异。
肝素和依诺肝素可降低大鼠妊娠时E-钙黏蛋白的表达，但不影响层粘连蛋白的表达。它们可能调节滋养层细胞的侵袭。我们认为，这可能是流产史患者使用肝素和低分子量肝素改善滋养层细胞侵袭的潜在机制。
