Deb Chitta Ranjan
Department of Botany, Nagaland University, Headquarters, Lumami, Mokokchung, India.
Indian J Exp Biol. 2006 Sep;44(9):762-6.
Rapid in vitro propagation of the terrestrial orchid, M. khasiana through immature seed culture was achieved. Immature seeds of 8-9 week after pollination (WAP) cultured on MS medium (2% sucrose) supplemented with 500 mgl(-1) casein-hydrolysate and 1 microM N6-benzyladenine (BA) exhibited germination of 75% seeds after 107 days of culture and subsequently supported the development of PLBs. Subsequent culture on MS medium enriched with 6 microM of indole-3-acetic acid (IAA), 18 microM each of BA and kinetin induced multiple shoots and plantlets. Transfer of PLBs to MS medium with 0.1% activated charcoal (AC) facilitated rapid proliferation of PLBs, while AC at 0.2% favored shoot bud induction and rhizome enlargement. The plantlets, developed on medium with IAA, BA and kinetin, after hardening in vitro for 8-10 weeks were planted in community pots and transferred to poly-house. The plantlets showed 65% survival under field conditions.
通过未成熟种子培养实现了地生兰花卡西兜唇兰的快速离体繁殖。授粉后8 - 9周（WAP）的未成熟种子在添加了500 mg/L水解酪蛋白和1 μM N6 - 苄基腺嘌呤（BA）的MS培养基（2%蔗糖）上培养，培养107天后种子发芽率达75%，随后支持了原球茎的发育。随后在添加6 μM吲哚 - 3 - 乙酸（IAA）、18 μM BA和激动素的MS培养基上培养诱导产生多个芽和小植株。将原球茎转移到含有0.1%活性炭（AC）的MS培养基上促进了原球茎的快速增殖，而0.2%的AC有利于芽诱导和根状茎增大。在含有IAA、BA和激动素的培养基上发育的小植株，在体外硬化8 - 10周后种植在社区花盆中并转移到温室。这些小植株在田间条件下的存活率为65%。
