Lee Yung-I
Botany Department, National Museum of Natural Science, Taiwan, ROC.
Methods Mol Biol. 2011;710:53-62. doi: 10.1007/978-1-61737-988-8_5.
Orchidaceae is one of the largest families of flowering plants, and many of its species are highly valuable as herbal medicines and to the horticultural industry. To meet commercial requirements and to conserve natural resources, in vitro seed germination has been utilized to produce large quantities of uniform seedlings. In general, terrestrial orchid seeds are more difficult to germinate and grow than epiphytic orchids. Terrestrial orchid seeds have a hardened seed coat and more stringent requirements for germination in vitro. In this chapter, we document the timing of seed collection and pretreatments for improving in vitro germination of some terrestrial Asian orchids. The process of in vitro germination is demonstrated, including (1) the culture of immature seeds; (2) the culture of mature seeds; and (3) subsequent seedling development. For immature seed culture, optimal timing of seed harvest is key to maximizing germination; for mature seed culture, selection of adequate pretreatment conditions (i.e., the duration and concentrations of pretreatment solutions) is essential to improve germination.
兰科是开花植物中最大的科之一，其许多物种作为草药和对园艺产业具有极高价值。为满足商业需求并保护自然资源，已利用离体种子萌发来生产大量均匀一致的幼苗。一般而言，地生兰花种子比附生兰花种子更难萌发和生长。地生兰花种子具有坚硬的种皮，对离体萌发有更严格的要求。在本章中，我们记录了一些亚洲地生兰花种子收集的时间和预处理方法，以提高其离体萌发率。展示了离体萌发的过程，包括：（1）未成熟种子的培养；（2）成熟种子的培养；以及（3）后续的幼苗发育。对于未成熟种子培养，种子收获的最佳时间是使萌发率最大化的关键；对于成熟种子培养，选择合适的预处理条件（即预处理溶液的持续时间和浓度）对于提高萌发率至关重要。
