Giudice L C, Farrell E M, Pham H, Rosenfeld R G
Department of Gynecology and Obstetrics, Stanford University Medical Center, California 94305.
J Clin Endocrinol Metab. 1990 Nov;71(5):1330-8. doi: 10.1210/jcem-71-5-1330.
Two major insulin-like growth factor-binding proteins, IGFBP-2 and IGFBP-3, were identified by Western ligand blotting of human follicular fluid (FF) aspirated from luteinizing follicles during ovarian stimulation with human menopausal gonadotropin (LH and FSH). IGFBP-1 was also present in FF, and an IGFBP with a mol wt of 24000 daltons, present in serum, appeared to be absent in FF. Identification of IGFBP-1, IGFBP-2, and IGFBP-3 was confirmed using IGFBP-specific antisera. IGF-I and IGF-II levels were measured in patient serum and FF at the time of oocyte harvest, and serum levels of both peptides were consistently higher than FF levels. The finding of three major serum IGFBPs in human FF suggests that IGF regulation in the ovary is probably under complex control by this set of regulatory BPs.
在用人类绝经期促性腺激素（促黄体生成素和促卵泡激素）刺激卵巢期间，从成熟卵泡中抽吸的人卵泡液（FF）经蛋白质免疫印迹法鉴定出两种主要的胰岛素样生长因子结合蛋白，即IGFBP - 2和IGFBP - 3。IGFBP - 1也存在于卵泡液中，而血清中存在的一种分子量为24000道尔顿的IGFBP在卵泡液中似乎不存在。使用IGFBP特异性抗血清证实了IGFBP - 1、IGFBP - 2和IGFBP - 3的鉴定。在取卵时测定了患者血清和卵泡液中的IGF - I和IGF - II水平，两种肽的血清水平始终高于卵泡液水平。在人卵泡液中发现三种主要的血清IGFBP表明，卵巢中的IGF调节可能受这组调节性结合蛋白的复杂控制。
