Temel Yasin, Visser-Vandewalle Veerle, Kaplan Süleyman, Kozan Ramazan, Daemen Marc A R C, Blokland Arjan, Schmitz Christoph, Steinbusch Harry W M
Department of Psychiatry and Neuropsychology, Division of Cellular Neurosciences, Maastricht University, and Department of Neurosurgery, University Hospital Maastricht, Maastricht, The Netherlands.
Brain Res. 2006 Nov 20;1120(1):100-5. doi: 10.1016/j.brainres.2006.08.082. Epub 2006 Sep 26.
In Parkinson disease (PD), the subthalamic nucleus (STN) becomes hyperactive (disinhibited), which is reported to cause excitotoxic damage to midbrain dopaminergic neurons. Here, we examined whether silencing of the hyperactive STN by chronic bilateral deep brain stimulation (DBS) increased the survival of midbrain dopaminergic neurons in a rat model of PD. High-precision design-based stereologic examination of the total number of neurons and tyrosine tydroxylase (TH) immunoreactive neurons in the substantia nigra pars compacta revealed that STN DBS resulted in a significant survival of these neurons. These data provide the first evidence in vivo that bilateral STN DBS is useful for protecting midbrain dopaminergic neurons from cell death in PD.
在帕金森病（PD）中，底丘脑核（STN）变得过度活跃（去抑制），据报道这会导致对中脑多巴胺能神经元的兴奋性毒性损伤。在此，我们研究了通过慢性双侧深部脑刺激（DBS）使过度活跃的STN沉默是否能增加PD大鼠模型中脑多巴胺能神经元的存活率。基于高精度设计的体视学检查黑质致密部中神经元总数和酪氨酸羟化酶（TH）免疫反应性神经元，结果显示STN DBS导致这些神经元显著存活。这些数据提供了首个体内证据，表明双侧STN DBS有助于保护PD中脑多巴胺能神经元免于细胞死亡。
