多聚腺苷酸对脊髓灰质炎病毒核糖核酸功能的研究。

Studies on the function of polyadenylic acid on poliovirus RNA.

作者信息

Spector D H, Villa-Komaroff L, Baltimore D

出版信息

Cell. 1975 Sep;6(1):41-4. doi: 10.1016/0092-8674(75)90071-9.

Abstract

RNAase H was used to remove selectively over 83% of 3' terminal polyadenylic acid [poly(A)] from poliovirus RNA. In a single cycle infection with this poly(A)-deficient RNA, no evidence for multiplication of the RNA was found. To elucidate further the function of poliovirus poly (A), the translational capacity of the RNAase H-treated and untreated RNA was studied in a cell-free protein synthesizing system prepared from HeLa cells. Removal of most of the poly(A) did not affect the pattern or amount of polypeptides synthesized. We conclude that the poly(A) on poliovirus RNA is probably necessary to the replication of the molecule.

摘要

核糖核酸酶H被用于从脊髓灰质炎病毒RNA中选择性去除超过83%的3'末端多聚腺苷酸[poly(A)]。用这种缺乏多聚腺苷酸的RNA进行单次循环感染时，未发现RNA增殖的证据。为了进一步阐明脊髓灰质炎病毒多聚腺苷酸的功能，在由HeLa细胞制备的无细胞蛋白质合成系统中研究了经核糖核酸酶H处理和未处理的RNA的翻译能力。去除大部分多聚腺苷酸并不影响合成的多肽的模式或数量。我们得出结论，脊髓灰质炎病毒RNA上的多聚腺苷酸可能对该分子的复制是必需的。