脊髓灰质炎病毒RNA感染性对3'-末端聚腺苷酸的需求
Requirement of 3'-terminal poly(adenylic acid) for the infectivity of poliovirus RNA.
作者信息
Spector D H, Baltimore D
出版信息
Proc Natl Acad Sci U S A. 1974 Aug;71(8):2983-7. doi: 10.1073/pnas.71.8.2983.
Abstract
Ribonuclease H (EC 3.1.4.34) has been used to remove selectively much of the 3'-terminal poly(adenylic acid) [poly(A)] from poliovirus RNA by treating the RNA with the enzyme in the presence of poly(dT). Over 80% of the poly(A) could be removed and the residuum was found as oligo(A) stretches attached to many or all of the viral RNA molecules. Reduction of the size of the poly(A) markedly decreased the specific infectivity of poliovirus RNA, indicating that poly(A) is necessary to the infectivity of the RNA. The virions in plaques deriving from infection with treated RNA have a normal amount and size of poly(A), indicating that mechanisms exist in infected cells to regenerate normal length poly(A) from truncated poly(A).
摘要
核糖核酸酶H（EC 3.1.4.34）已被用于通过在聚（dT）存在下用该酶处理脊髓灰质炎病毒RNA来选择性地去除其大部分3'-末端聚（腺苷酸）[聚（A）]。超过80%的聚（A）可以被去除，并且残余物被发现是附着在许多或所有病毒RNA分子上的寡聚（A）片段。聚（A）大小的减小显著降低了脊髓灰质炎病毒RNA的比感染性，表明聚（A）对于RNA的感染性是必需的。源自用处理过的RNA感染所形成噬斑中的病毒粒子具有正常数量和大小的聚（A），这表明在受感染细胞中存在从截短的聚（A）再生正常长度聚（A）的机制。
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